Pan-TEAD (D3F7L) Rabbit mAb

• 产品编号：CST-13295S      品牌：CST       原厂货号：13295S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Pan-TEAD (D3F7L) Rabbit mAb兔单抗可以识别内源性的总TEAD蛋白。该抗体可以识别转染后细胞中的TEAD1,2,3和4。单克隆抗体由合成肽段免疫动物产生，该肽段与人TEAD1蛋白Gly54邻近氨基酸残基序列一致。Hippo通路是一个重要的进化保守的信号通路，通过抑制细胞增殖促进凋亡以控制器官的大小和抑制肿瘤（1,2）。Hippo通路的完整功能是抑制Yes-相关蛋白(YAP)的活性，这是一个致癌基因，其活性受到磷酸化和亚细胞定位的调控（3,4）。当Hippo通路被关闭后，YAP被磷酸化并转位到细胞核，YAP与多种转录因子包括转录增强因子（TEF）家族相互作用，如TEA结构域（TEAD）家族(TEAD1-4) (5,6)。尽管在组织中表达广泛，TEAD家族蛋白有组织特异性和发育特异性。YAP/TEAD复合物调控细胞增殖和凋亡过程中的基因表达（5）。

1. Pan, D. (2010) Dev Cell 19, 491-505.
2. Harvey, K.F. et al. (2003) Cell 114, 457-67.
3. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
4. Zhao, B. et al. (2008) Curr Opin Cell Biol 20, 638-46.
5. Zhao, B. et al. (2008) Genes Dev 22, 1962-71.
6. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.

原厂资料：

Specificity / Sensitivity

Pan-TEAD (D3F7L) Rabbit mAb recognizes endogenous levels of total TEAD proteins. This antibody has been shown to recognize TEAD 1, 2, 3 and 4 in transfected cell extracts.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly54 of human TEAD1 protein.

Background

The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of Yes-associated protein (YAP), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). When the Hippo pathway is turned off, YAP is phosphorylated and translocates to the nucleus where it associates with various transcription factors including members of the transcriptional enhancer factor (TEF) family, also known as the TEA domain (TEAD) family (TEAD1-4) (5,6). Although widely expressed in tissues, the TEAD family proteins have specific tissue and developmental distributions. YAP/TEAD complexes regulate the expression of genes involved in cell proliferation and apoptosis (5).

1. Pan, D. (2010) Dev Cell 19, 491-505.
2. Harvey, K.F. et al. (2003) Cell 114, 457-67.
3. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
4. Zhao, B. et al. (2008) Curr Opin Cell Biol 20, 638-46.
5. Zhao, B. et al. (2008) Genes Dev 22, 1962-71.
6. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody