Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb

• 产品编号：CST-13281S      品牌：CST       原厂货号：13281S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb 兔单抗仅能识别Ser518磷酸化的内源性的merlin蛋白。该单克隆抗体由合成的人merlin蛋白Ser518位点附近肽段免疫动物而获得。神经纤维瘤病2（NF2）是一种常染色体显性遗传病，以前庭神经鞘瘤，脑膜瘤和其他神经系统肿瘤的发生为特点。NF2家族性肿瘤患者和人群中散发的肿瘤，都是由NF2肿瘤抑制基因的失活引起。Merlin (moesin, ezrin和radixin样蛋白)都是NF2基因的产物，与ezrin, radixin和moesin (ERM)有惊人的相似性。merlin (aka schwannomin) and ERM蛋白的调控涉及家族成员中分子内部及分子间的头尾关系（1）。Merlin和ERM蛋白的功能是作为细胞膜和细胞骨架之间的连接，影响细胞的形态，极性和信号转导（2）。Rac/Cdc42的效应器p21激活的激酶（PAK）可以磷酸化Merlin的Ser518，负向调节Rac（3,4）。

1. Ramesh, V. (2004) Nat. Rev. Neurosci. 5, 462-470.
2. Bretscher, A. et al. (2002) Nat. Rev. Mol. Cell Biol. 3, 586-599.
3. Xiao, G. H. et al. (2002) J. Biol. Chem. 277, 883-886.
4. Kissil, J. L. et al. (2003) Mol. Cell 12, 841-849.

原厂资料：

Specificity / Sensitivity

Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb recognizes endogenous levels of merlin protein only when phosphorylated at Ser518.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser518 of human merlin protein.

Background

Neurofibromatosis 2 (NF2) is an autosomal dominant, inherited disorder characterized by the occurrence of vestibular schwannomas, meningiomas, and other nervous system tumors. Both the familial tumors of NF2 and equivalent sporadic tumors found in the general population are caused by inactivation of the NF2 tumor suppressor gene. Merlin (moesin, ezrin, and radixin-like protein) is the NF2 gene product, displaying striking similarity to ezrin, radixin, and moesin (ERM) proteins. Regulation of merlin (also called schwannomin) and ERM proteins involves intramolecular and intermolecular head-to-tail associations between family members (1). Merlin and ERM proteins act as linkers between the plasma membrane and the cytoskeleton, affecting cell morphology, polarity, and signal transduction (2). Merlin is phosphorylated by the Rac/Cdc42 effector p21-activated kinase (PAK) at Ser518, negatively regulating Rac (3,4).

1. Ramesh, V. (2004) Nat. Rev. Neurosci. 5, 462-470.
2. Bretscher, A. et al. (2002) Nat. Rev. Mol. Cell Biol. 3, 586-599.
3. Xiao, G. H. et al. (2002) J. Biol. Chem. 277, 883-886.
4. Kissil, J. L. et al. (2003) Mol. Cell 12, 841-849.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.