ATPIF1 (D6P1Q) XP? Rabbit mAb

• 产品编号：CST-13268P      品牌：Cell Signaling Technology       原厂货号：13268P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ATPIF1 (D6P1Q) XP ® Rabbit mAb兔单抗可以识别内源性ATPIF1总蛋白水平。ATPIF1 (D6P1Q) XP ® Rabbit mAb兔单抗可以识别内源性ATPIF1总蛋白水平。ATP酶抑制因子1（ATPIF1）基因编码线粒体ATP酶抑制剂，该抑制剂可限制线粒体呼吸受损时ATP耗竭（1）。ATPIF1在pH降低时被激活，结合β-F1-ATPase，抑制H-ATP合成酶的水解活性（1,2）。ATPIF1除了作为一个ATP水解酶的作用，也被证明在细胞能量代谢中发挥调节作用，它可以引发癌细胞的有氧糖酵解导致其Warburg表型（3,4）。在几个人类肿瘤中过度表达的ATPIF1进一步支持其参与肿瘤发生，并改变了癌细胞的新陈代谢，包括重编程代谢模式至糖酵解（3）。

1. Gledhill, J.R. et al. (2007) Proc Natl Acad Sci USA 104, 15671-6.
2. Cabezón, E. et al. (2003) Nat Struct Biol 10, 744-50.
3. Sánchez-Cenizo, L. et al. (2010) J Biol Chem 285, 25308-13.
4. Wallace, D.C. (2005) Cold Spring Harb Symp Quant Biol 70, 363-74.

原厂资料：

Specificity / Sensitivity

ATPIF1 (D6P1Q) XP^® Rabbit mAb recognizes endogenous levels of total ATPIF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala46 of human ATPIF1 protein.

Background

The ATPase inhibitor factor 1 (ATPIF1) gene encodes a mitochondrial ATPase inhibitor that limits ATP depletion when mitochondrial respiration is impaired (1). ATPIF1 becomes activated following a drop in pH, binding to β-F1-ATPase, thereby inhibiting the hydrolase activity of the H⁺-ATP synthase (1,2). In addition to its role as an ATP hydrolase, ATPIF1 has also been shown to play a regulatory role in cellular energy metabolism by triggering the induction of aerobic glycolysis in cancer cells resulting in their Warburg phenotype (3,4). Research studies demonstrate that the overexpression of ATPIF1 in several human carcinomas further supports its participation in oncogenesis and provides insight into the altered metabolism of cancer cells, which includes the reprogramming of energetic metabolism toward glycolysis (3).

1. Gledhill, J.R. et al. (2007) Proc Natl Acad Sci USA 104, 15671-6.
2. Cabezón, E. et al. (2003) Nat Struct Biol 10, 744-50.
3. Sánchez-Cenizo, L. et al. (2010) J Biol Chem 285, 25308-13.
4. Wallace, D.C. (2005) Cold Spring Harb Symp Quant Biol 70, 363-74.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody