CtBP2 Antibody

• 产品编号：CST-13256S      品牌：CST       原厂货号：13256S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CtBP2抗体可以识别内源性的总CtBp2蛋白。该抗体与CtBP1没有交叉反应。多抗由合成肽段免疫动物产生，该肽段与人CtBP2蛋白羧基末端附近氨基酸序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。CtBP2 (carboxy-terminal binding protein-2)和同源蛋白CtBP1是转录共抑制因子，最初因其能与人腺病毒E1A蛋白的羧基末端结合而被发现（1-3）。CtBP蛋白被认为在调控多条信号通路过程中发挥了重要作用，因为敲除CtBP2会导致E10.5的胚胎致死，以及和轴向发育模式缺陷相关（4）。CtBP蛋白调控多种肿瘤信号通路作为上皮-间质转换的启动子，细胞凋亡拮抗剂和肿瘤抑制基因的抑制因子(1,5)。CtBP蛋白转录共抑制因子活性来自于和多种转录因子以及染色质调控子，包括聚梳家族蛋白(1,6,7)。CtBP蛋白与一个短的氨基酸序列基序(PXDLS)结合通过组蛋白去乙酰依赖和非依赖通路介导对靶基因的抑制效果(6,8,9)。CtBP蛋白与细菌D-isomer-specific 2-hydroxyacid dehydrogenase酶有高度序列同源性。研究表明核NAPDH水平调控CtBP转录抑制功能，因为NAPDH的结合对CtBP2的同二聚化和转录共抑制活性(6,9-11)。

1. Chinnadurai, G. (2009) Cancer Res 69, 731-4.
2. Boyd, J.M. et al. (1993) EMBO J 12, 469-78.
3. Katsanis, N. and Fisher, E.M. (1998) Genomics 47, 294-9.
4. Hildebrand, J.D. and Soriano, P. (2002) Mol Cell Biol 22, 5296-307.
5. Battaglia, S. et al. (2010) Int J Cancer 126, 2511-9.
6. Chinnadurai, G. (2002) Mol Cell 9, 213-24.
7. Sewalt, R.G. et al. (1999) Mol Cell Biol 19, 777-87.
8. Molloy, D.P. et al. (1998) J Biol Chem 273, 20867-76.
9. Schaeper, U. et al. (1995) Proc Natl Acad Sci U S A 92, 10467-71.
10. Kumar, V. et al. (2002) Mol Cell 10, 857-69.
11. Thio, S.S. et al. (2004) Nucleic Acids Res 32, 1836-47.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Guinea Pig

Specificity / Sensitivity

CtBP2 Antibody recognizes endogenous levels of total CtBP2 protein. This antibody does not cross-react with the CtBP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CtBP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CtBP2 (carboxy-terminal binding protein-2) and its homolog CtBP1 are transcriptional co-repressors originally identified as proteins that bind the carboxy-terminus of the human adenovirus E1A protein (1-3). CtBP proteins are thought to play important roles in regulating various developmental pathways because deletion of CtBP2 leads to embryonic lethality at E10.5 and is correlated with axial patterning defects (4). CtBP proteins regulate various oncogenic signaling pathways as promoters of epithelial-mesenchymal transition, apoptosis antagonists, and tumor suppressor genes repressors (1,5). The CtBP protein transcription co-repression activity results from interactions with numerous transcription factors and chromatin modulators, including the polycomb group proteins (1,6,7). Depending on the context, CtBP proteins interact with a short amino acid sequence motif (PXDLS) to mediate repression of target genes through both histone deacetylase-dependent and independent mechanisms (6,8,9). CtBP proteins display a high sequence homology to the bacterial D-isomer-specific 2-hydroxyacid dehydrogenase enzymes. Research studies indicate that nuclear NADH levels regulate CtBP transcription repression activities, as NADH binding is required for CtBP2 homodimerization and transcription co-repressor activity (6,9-11).

1. Chinnadurai, G. (2009) Cancer Res 69, 731-4.
2. Boyd, J.M. et al. (1993) EMBO J 12, 469-78.
3. Katsanis, N. and Fisher, E.M. (1998) Genomics 47, 294-9.
4. Hildebrand, J.D. and Soriano, P. (2002) Mol Cell Biol 22, 5296-307.
5. Battaglia, S. et al. (2010) Int J Cancer 126, 2511-9.
6. Chinnadurai, G. (2002) Mol Cell 9, 213-24.
7. Sewalt, R.G. et al. (1999) Mol Cell Biol 19, 777-87.
8. Molloy, D.P. et al. (1998) J Biol Chem 273, 20867-76.
9. Schaeper, U. et al. (1995) Proc Natl Acad Sci U S A 92, 10467-71.
10. Kumar, V. et al. (2002) Mol Cell 10, 857-69.
11. Thio, S.S. et al. (2004) Nucleic Acids Res 32, 1836-47.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody