GPNMB (E1Y7J) Rabbit mAb

• 产品编号：CST-13251S      品牌：CST       原厂货号：13251S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GPNMB (E1Y7J) Rabbit mAb可以识别内源性总的GPNMB蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人GPNMB蛋白Asp247附近氨基酸序列一致。Glycoprotein non-metastatic gene B (GPNMB)是一个在多种癌症中共表达的1型跨膜糖蛋白。GPNMB糖蛋白参与调控很多生理过程，包括介导晚期黑色素转运到角质形成细胞(1)，调节城固细胞和破骨细胞的分化和功能(2)，刺激树突细胞成熟，促进树突状细胞黏附到内皮细胞(3),提高自噬体融合到组织修复的溶酶体中，并调控细胞碎片的降解。GPNMB一般表达在皮肤，心脏，肾脏，肺，肝和骨骼肌中(3,6)，研究显示在许多癌症中GPNMB高表达旺旺有助于转移(reviewed in 7)。正常细胞中，GPNMB位于细胞内结构(1,8),但是研究人员发现癌细胞中它主要位于细胞表面(9,10)。位置和表达水平的不同，以及许多癌症中GPNMB的癌症推动因子的作用使这个蛋白可以作为治疗的靶点(11)。GPNMB胞外区域可以通过基质金属蛋白酶裂解并从细胞表面脱落(12)。研究确认sheddase ADAM10可以作为肽酶使GPNMB胞外区从乳腺癌细胞表面脱落。脱落的GPNMB胞外区可能通过诱导内皮细胞迁移促进血管生成(13)。

1. Tomihari, M. et al. (2009) Exp Dermatol 18, 586-95.
2. Sheng, M.H. et al. (2012) PLoS One 7, e35280.
3. Shikano, S. et al. (2001) J Biol Chem 276, 8125-34.
4. Li, B. et al. (2010) FASEB J 24, 4767-81.
5. Patel-Chamberlin, M. et al. (2011) Kidney Int 79, 1138-48.
6. Bandari, P.S. et al. (2003) Regul Pept 111, 169-78.
7. Maric, G. et al. (2013) Onco Targets Ther 6, 839-52.
8. Ripoll, V.M. et al. (2007) J Immunol 178, 6557-66.
9. Tse, K.F. et al. (2006) Clin Cancer Res 12, 1373-82.
10. Rose, A.A. et al. (2010) Clin Cancer Res 16, 2147-56.
11. Keir, C.H. and Vahdat, L.T. (2012) Expert Opin Biol Ther 12, 259-63.
12. Furochi, H. et al. (2007) FEBS Lett 581, 5743-50.
13. Rose, A.A. et al. (2010) PLoS One 5, e12093.

原厂资料：

Specificity / Sensitivity

GPNMB (E1Y7J) Rabbit mAb recognizes endogenous levels of total GPNMB protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp247 of human GPNMB protein.

Background

Glycoprotein non-metastatic gene B (GPNMB) is a type I transmembrane glycoprotein over expressed in many types of cancer. The GPNMB glycoprotein is involved in many physiological processes, including mediating transport of late melanosomes to keratinocytes (1), regulating osteoblast and osteoclast differentiation and function (2), stimulating dendritic cell maturation, promoting adhesion of dendritic cells to endothelial cells (3), enhancing autophagosome fusion to lysomes in tissue repair, and regulating degradation of cellular debris (4,5).

While typical GPNMB expression is seen in tissues including skin, heart, kidney, lung, liver, and skeletal muscle (3,6), research studies show elevated GPNMB expression often contributes to the metastatic phenotype in numerous cancers (reviewed in 7). GPNMB is typically localized to intracellular compartments in normal cells (1,8), but investigators found it primarily on the cell surface of tumor cells (9,10). Differential localization and expression, and the role of GPNMB as a tumor promoter in many cancer types make this protein a viable therapeutic target (11).

The GPNMB ectodomain can be cleaved by matrix metalloproteinases and shed from the cell surface (12). Research studies identify the sheddase ADAM10 as one peptidase responsible for cleavage of the GPNMB ectodomain at the surface of breast cancer cells. Shedded GPNMB ectodomains may promote angiogenesis by inducing endothelial cell migration (13).

1. Tomihari, M. et al. (2009) Exp Dermatol 18, 586-95.
2. Sheng, M.H. et al. (2012) PLoS One 7, e35280.
3. Shikano, S. et al. (2001) J Biol Chem 276, 8125-34.
4. Li, B. et al. (2010) FASEB J 24, 4767-81.
5. Patel-Chamberlin, M. et al. (2011) Kidney Int 79, 1138-48.
6. Bandari, P.S. et al. (2003) Regul Pept 111, 169-78.
7. Maric, G. et al. (2013) Onco Targets Ther 6, 839-52.
8. Ripoll, V.M. et al. (2007) J Immunol 178, 6557-66.
9. Tse, K.F. et al. (2006) Clin Cancer Res 12, 1373-82.
10. Rose, A.A. et al. (2010) Clin Cancer Res 16, 2147-56.
11. Keir, C.H. and Vahdat, L.T. (2012) Expert Opin Biol Ther 12, 259-63.
12. Furochi, H. et al. (2007) FEBS Lett 581, 5743-50.
13. Rose, A.A. et al. (2010) PLoS One 5, e12093.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.