T7-Tag (D9E1X) XP? Rabbit mAb

• 产品编号：CST-13246P      品牌：Cell Signaling Technology       原厂货号：13246P
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

T7-Tag (D9E1X) XP^® Rabbit mAb能检测转染的包含T7附加表位的蛋白。单克隆抗体通过合成T7肽免疫动物制备。附加表位对于用免疫印迹、免疫沉淀和免疫染色技术来进行蛋白质的标记和检测很有用。因为它们很小，不大可能会影响被标记蛋白的生化特性。T7-tag是一个表位肽标签，拥有从T7噬菌体基因编码而来的11个残基。这种肽可以广泛地运用到许多类似于pET系统的表达载体中，这个系统是基于T7RNA多聚酶表达。

1. Studier, F.W. and Moffatt, B.A. (1986) J Mol Biol 189, 113-30.

原厂资料：

Specificity / Sensitivity

T7-Tag (D9E1X) XP^® Rabbit mAb recognizes transfected levels of recombinant protein containing the T7 epitope tag.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic T7-tag peptide.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems (1).

1. Studier, F.W. and Moffatt, B.A. (1986) J Mol Biol 189, 113-30.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.