Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb?(PE Conjugate)

• 产品编号：CST-13223S      品牌：CST       原厂货号：13223S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Pig

Specificity / Sensitivity

Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Stat4 protein only when phosphorylated at Tyr693.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr693 of human Stat4 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb #4134.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Pig

Specificity / Sensitivity

Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Stat4 protein only when phosphorylated at Tyr693.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr693 of human Stat4 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Stat4 (Tyr693) (D2E4) Rabbit mAb #4134.

Background

The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9).

1. Darnell, J.E. et al. (1994) Science 264, 1415-1421.
2. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
3. Zhong, Z. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4806-4810.
4. Yamamoto, K. et al. (1994) Mol. Cell Biol. 14, 4342-4349.
5. Jacobson, N.G. et al. (1995) J. Exp. Med. 181, 1755-1762.
6. Bacon, C.M. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 7307-7311.
7. Thierfelder, W.E. et al. (1996) Nature 382, 171-174.
8. Kaplan, M.H. et al. (1996) Nature 382, 174-177.
9. Visconti, R. et al. (2000) Blood 96, 1844-52.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibod-
ies. Protect from light. Do not freeze.