SignalSilence? SYVN1 siRNA I

• 产品编号：CST-13204S      品牌：CST       原厂货号：13204S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence^® SYVN1 siRNA I抑制人、猴中SYVN1表达。来自Cell Signaling Technology (CST)的SignalSilence^® SYVN1 siRNA I可以帮助研究者通过RNA干扰特异性地抑制 SYVN1的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^®SYVN1 siRNA I 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。每小瓶可供100次转染，每次转染量相当于在转染24孔板时，每孔总体积为300μl培养基中siRNA的终浓度为100nM。Synoviolin-1(SYVN1 / HRD1)是一个环形E3泛素蛋白连接酶和内质网(ER)质量控制系统的主要组件，参与错误折叠蛋白泛素依赖性的降解 (1)。SYVN1 是一个多次跨膜的ER蛋白，在ER应激条件下，其蛋白表达量上调(1 - 4)。研究表明SYVN1是一种与关节病的发病机制相关的抗凋亡因子，促进滑膜增生(5)。此外,基因靶向研究已经证明,SYVN1表达式是胚胎发生中必不可少(6)。

1. Kikkert, M. et al. (2004) J Biol Chem 279, 3525-34.
2. Kaneko, M. et al. (2007) FEBS Lett 581, 5355-60.
3. Yamamoto, K. et al. (2008) J Biochem 144, 477-86.
4. Nadav, E. et al. (2003) Biochem Biophys Res Commun 303, 91-7.
5. Amano, T. et al. (2003) Genes Dev 17, 2436-49.
6. Yagishita, N. et al. (2005) J Biol Chem 280, 7909-16.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

SignalSilence^® SYVN1 siRNA I inhibits human and monkey SYVN1 expression.

Description

SignalSilence^® SYVN1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit SYVN1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence^® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence^® SYVN1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

Synoviolin-1 (SYVN1/HRD1) is a RING-type E3 ubiquitin-protein ligase and major component of the endoplasmic reticulum (ER) quality control system that is involved in the ubiquitin-dependent degradation of misfolded proteins (1). SYVN1 is a multispanning ER membrane protein whose expression is upregulated at the protein level under conditions that promote ER stress (1-4). Research studies have shown that SYVN1 is an anti-apoptotic factor that is implicated in the pathogenesis of arthropathy by promoting synovial hyperplasia (5). Furthermore, gene-targeting studies have demonstrated that SYVN1 expression is indispensable for embryogenesis (6).

1. Kikkert, M. et al. (2004) J Biol Chem 279, 3525-34.
2. Kaneko, M. et al. (2007) FEBS Lett 581, 5355-60.
3. Yamamoto, K. et al. (2008) J Biochem 144, 477-86.
4. Nadav, E. et al. (2003) Biochem Biophys Res Commun 303, 91-7.
5. Amano, T. et al. (2003) Genes Dev 17, 2436-49.
6. Yagishita, N. et al. (2005) J Biol Chem 280, 7909-16.

注意事项：

Storage: SYVN1 siRNA I is supplied in RNAse-free water.
Aliquot and store at -20ºC.