AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb

• 产品编号：CST-13185S      品牌：CST       原厂货号：13185S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb 兔单抗可以识别内源性的AMPA Receptor (GluR 1)总蛋白。该单克隆抗体由合成肽段免疫动物产生，该肽段与人AMPA Receptor (GluR 1)蛋白Ala275附近氨基酸一致。AMPA（α-氨基-3-羟基-5-甲基-4-恶唑酸），kainite和NMDA（N-甲基-D-天冬氨酸）受体是离子型谷氨酸门控离子通道的三个主要的家族。AMPA受体（AMPARs）是由四个亚基（GluR1-4）构成，以同源或异源四聚体的形式介导大多数中枢神经系统的兴奋性传递。AMPARs与突触的形成，稳定和可塑性有关(1)。相对于含有GluR-2的AMPARs，缺少GluR2的AMPARs对钙离子是可渗透的(2)。转录后修饰（剪接，RNA编辑核）和翻译后修饰（糖基化，磷酸化）可以导致结果的多样，可以微调AMPARs的动力学性质。研究表明多种疾病中AMPARs的功能会发生变化，包括阿尔茨海默氏症，肌萎缩侧索硬化症（ALS），中风，和癫痫(1)。GluR 1 对海马长时程增强(LTP)的表达和短期记忆的形成是必需的(3)。海马GluR1也参与了吗啡诱导的适应性突触机制(4)。

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog

Specificity / Sensitivity

AMPA Receptor (GluR 1) (D4N9V) Rabbit mAb recognizes endogenous levels of total AMPA Receptor (GluR 1) protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala275 of human AMPA Receptor (GluR 1) protein.

Background

AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluR 2-containing AMPARs, AMPARs that lack GluR 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).

GluR 1 is necessary for expression of long-term potentiation (LTP) in the hippocampus and formation of short-term memory (3). Hippocampal GluR 1 is also involved in morphine-induced adaptive synaptic mechanisms (4).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.