Phospho-GIT2 (Tyr592) (D6L1J) Rabbit mAb

• 产品编号：CST-13167S      品牌：CST       原厂货号：13167S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-GIT2 (Tyr592) (D6L1J) Rabbit mAb可以识别Tyr592被磷酸化的内源性总的GIT2蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人GIT2蛋白Tyr592附近氨基酸一致。G蛋白欧联受体(GPCR)激酶相互作用蛋白1和2(GIT1和GIT2)是高度保守，广泛表达的折叠蛋白，参与调控黏着斑装配和细胞骨架动态变化。GIT蛋白有多个相互作用结构域使其能和小GTPases结合(包括ARF，Rac和cdc42)，激酶（例如PAK和MEK），Rho家族GEF Pix，以及黏着斑蛋白paxillin（reviewed in 1）。GIT1和GIT2有很多相同点，但也有至少10个不同的，组织特异性的剪接异构体。GIT2能抑制黏着斑翻转和细胞膜突出物(2,3)。黏着斑位置以及paxillin和GIT2的结合是通过FAK和/或Src磷酸化GIT2的一个或多个酪氨酸位点来进行调控的（Tyr286, Tyr392, Tyr592）(4,5,reviewed in 6)。一旦位于黏着斑，GIT2可以在细胞极性和细胞迁移上发挥关键作用，使它成为癌症信号通路研究上一个让人有兴趣的蛋白（3,5,7）。

1. Hoefen, R.J. and Berk, B.C. (2006) J Cell Sci 119, 1469-75.
2. Premont, R.T. et al. (2000) J Biol Chem 275, 22373-80.
3. Frank, S.R. et al. (2006) EMBO J 25, 1848-59.
4. Brown, M.C. et al. (2005) Mol Biol Cell 16, 4316-28.
5. Yu, J.A. et al. (2009) Mol Biol Cell 20, 4706-19.
6. Yu, J.A. et al. (2010) Cell Adh Migr 4, 342-7.
7. Mazaki, Y. et al. (2006) Nat Immunol 7, 724-31.

原厂资料：

Specificity / Sensitivity

Phospho-GIT2 (Tyr592) (D6L1J) Rabbit mAb recognizes endogenous levels of GIT2 protein only when phosphorylated at Tyr592.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr592 of human GIT2 protein.

Background

G protein-coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac, and cdc42), kinases (such as PAK and MEK), the Rho family GEF Pix, and the focal adhesion protein paxillin (reviewed in 1). GIT1 and GIT2 share many of the same properties, but with at least ten distinct, tissue-specific splice variants. GIT2 has been shown to play an important role inhibiting focal adhesion turnover and membrane protrusion (2,3). Focal adhesion localization and paxillin binding of GIT2 is regulated through phosphorylation at one or more tyrosine sites (Tyr286, Tyr392, Tyr592) by FAK and/or Src (4,5,reviewed in 6). Once at the focal adhesion, GIT2 is thought to play a key role in cell polarity and migration, making it a protein of interest in the investigation of oncogenic signaling pathways (3,5,7).

1. Hoefen, R.J. and Berk, B.C. (2006) J Cell Sci 119, 1469-75.
2. Premont, R.T. et al. (2000) J Biol Chem 275, 22373-80.
3. Frank, S.R. et al. (2006) EMBO J 25, 1848-59.
4. Brown, M.C. et al. (2005) Mol Biol Cell 16, 4316-28.
5. Yu, J.A. et al. (2009) Mol Biol Cell 20, 4706-19.
6. Yu, J.A. et al. (2010) Cell Adh Migr 4, 342-7.
7. Mazaki, Y. et al. (2006) Nat Immunol 7, 724-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.