Integrin β3 (D7X3P) XP? Rabbit mAb

• 产品编号：CST-13166P      品牌：Cell Signaling Technology       原厂货号：13166P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Integrin β3 (D7X3P) XP® Rabbit mAb可以识别内源性总的整合素β3蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人整合素β3蛋白Ile114附近氨基酸序列一致。整合素是异二聚体细胞表面受体，在细胞黏附和迁移，以及生长和存活中发挥着重要的作用(1,2)。整合素家族含有至少18个α和8个β亚基形成了24种组织分布不同以及重叠的配体特异性的整合素(3)。整合素不仅将细胞外环境穿提到细胞内部(从外到内信号传递)，也检测到细胞内的信息以改变它们和细胞外环境的相互作用(从内向外信号传递) (1,2)。αIIβ3 和αVβ3是两个含有β3的整合素，主要表达在造血细胞和血管生成内皮细胞中，在止血，伤口愈合和血管生成过程中发挥了黏附功能(1,4). Tyr773 和 Tyr785（基于鸡的序列经常被认为是Tyr747和Tyr759）可以在结合配体后被磷酸化(6)。血小板特异性αIIβ3 中的整合素β3 的Thr779（基序列中的Thr753）可以被PKD以及/或Akt磷酸化，可能调节整合素与其他信号分子的结合。

1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
4. Shattil, S.J. (1995) Thromb Haemost 74, 149-55.
5. Blystone, S.D. (2002) J Biol Chem 277, 46886-90.
6. Butler, B. et al. (2003) J Biol Chem 278, 5264-70.
7. Kirk, R.I. et al. (2000) J Biol Chem 275, 30901-6.

原厂资料：

Specificity / Sensitivity

Integrin β3 (D7X3P) XP^® Rabbit mAb recognizes endogenous levels of total integrin β3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile114 of human integrin β3 protein.

Background

Integrins are heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). αIIβ3 and αVβ3 are the two β3 containing integrins which are prominently expressed in hematopoietic cells and angiogenic endothelic cells and perform adhesive functions in hemostasis, wound healing and angiogenesis (1,4). Tyr773 and Tyr785 (usually referred to as Tyr747 and Tyr759 based on the chicken sequence) are phosphorylated upon ligand binding (5). Phosphorylation of these tyrosine residues is required for certain ligand-induced signaling (6). Thr779 (corresponding to Thr753 of the chicken sequence) of integrin β3 in the platelet specific αIIβ3 is phosphorylated by PKD and/or Akt, which may modulate integrin association with other signaling molecules (7).

1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
4. Shattil, S.J. (1995) Thromb Haemost 74, 149-55.
5. Blystone, S.D. (2002) J Biol Chem 277, 46886-90.
6. Butler, B. et al. (2003) J Biol Chem 278, 5264-70.
7. Kirk, R.I. et al. (2000) J Biol Chem 275, 30901-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.