SOD2 (D3X8F) XP? Rabbit mAb

• 产品编号：CST-13141P      品牌：Cell Signaling Technology       原厂货号：13141P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SOD2 (D3X8F) XP® Rabbit mAb兔单抗可以识别内源性SOD2总蛋白水平。单克隆抗体由合成肽段免疫动物产生，该肽段与人SOD2蛋白氨基端附近氨基酸一致。锰超氧化物歧化酶（MnSOD或SOD2）是线粒体解毒酶，能将超氧化物催化转化为过氧化氢(1,2)。过氧化氢，随后通过过氧化氢酶，谷胱甘肽过氧化物酶，或过氧化物酶分解成水(2)。MnSOD/SOD2和其他酶参与抗氧化防御保护细胞免受活性氧(ROS)的伤害(2)。热量限制会导致SIRT3介导的MnSOD/SOD2去乙酰化随后增强其抗氧化能力(3)。MnSOD/SOD2还具有介导mTOR抑制的保护作用以减少上皮干细胞衰老的重要作用(4)。

1. Cooper, M.L. et al. (2008) Cancer Res 68, 10171-7.
2. Minig, V. et al. (2009) J Biol Chem 284, 14165-76.
3. Qiu, X. et al. (2010) Cell Metab 12, 662-7.
4. Iglesias-Bartolome, R. et al. (2012) Cell Stem Cell 11, 401-14.

原厂资料：

Specificity / Sensitivity

SOD2 (D3X8F) XP^® Rabbit mAb recognizes endogenous levels of total SOD2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SOD2 protein

Background

Manganese superoxide dismutase (MnSOD or SOD2) is a mitochondrial detoxification enzyme that catalyzes the conversion of superoxide to hydrogen peroxide (1,2). Hydrogen peroxide is then decomposed to water by catalase, glutathione peroxidase, or peroxiredoxins (2). MnSOD/SOD2 and other enzymes involved in antioxidant defense protect cells from reactive oxygen species (ROS) (2). Calorie restriction leads to SIRT3-mediated deacetylation of MnSOD/SOD2 and the subsequent increase of its antioxidant activity (3). MnSOD/SOD2 also plays an essential role in mediating the protective effect of mTOR inhibition to reduce epithelial stem cell senescence (4).

1. Cooper, M.L. et al. (2008) Cancer Res 68, 10171-7.
2. Minig, V. et al. (2009) J Biol Chem 284, 14165-76.
3. Qiu, X. et al. (2010) Cell Metab 12, 662-7.
4. Iglesias-Bartolome, R. et al. (2012) Cell Stem Cell 11, 401-14.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody