PathScan? Th1/Th2/Th17 Cytokine Antibody Array Kit (Fluorescent Readout)

• 产品编号：CST-13124S      品牌：CST       原厂货号：13124S
• 产品分类：ELISA及其它免疫分析 > 其它免疫检测 > 抗体芯片（Antibody Array)
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描述：

Specificity / Sensitivity

PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Fluorescent Readout) detects the target proteins as specified on the Array Target Map (figure 1). No significant cross reactivity has been observed between targets. This kit is optimized for cell culture supernatants. Recommended starting cell culture supernatant range is 20-75 μl. All antibodies have been validated for human-derived cell culture supernatants.

Description

Description: The PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Fluorescent Readout) uses glass slides as the planar surface and is based upon the sandwich immunoassay principle. This array kit allows for the simultaneous detection of 12 unique extracellular signaling molecules. Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two slides allowing the user to test up to 32 different samples and generate 384 data points in a single experiment. Cell supernatant is incubated on the slide followed by a biotinylated detection antibody cocktail. Streptavidin-conjugated Dylight™ 680 is then used to visualize the bound detection antibody. A fluorescent image of the slide can then be captured with a digital imaging system and spot intensities quantified using array analysis software.

原厂资料：

Specificity / Sensitivity

PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Fluorescent Readout) detects the target proteins as specified on the Array Target Map (figure 1). No significant cross reactivity has been observed between targets. This kit is optimized for cell culture supernatants. Recommended starting cell culture supernatant range is 20-75 μl. All antibodies have been validated for human-derived cell culture supernatants.

Description

Description: The PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit (Fluorescent Readout) uses glass slides as the planar surface and is based upon the sandwich immunoassay principle. This array kit allows for the simultaneous detection of 12 unique extracellular signaling molecules. Target-specific capture antibodies have been spotted in duplicate onto nitrocellulose-coated glass slides. Each kit contains two slides allowing the user to test up to 32 different samples and generate 384 data points in a single experiment. Cell supernatant is incubated on the slide followed by a biotinylated detection antibody cocktail. Streptavidin-conjugated Dylight™ 680 is then used to visualize the bound detection antibody. A fluorescent image of the slide can then be captured with a digital imaging system and spot intensities quantified using array analysis software.

ackground

Cytokines are secreted intercellular signaling molecules that regulate many biological processes including inflammation, host defense, and cell differentiation. Cytokine profiles may provide insight into the molecular mechanisms that distinguish between healthy and diseased states. The PathScan^® Th1/Th2/Th17 Cytokine Antibody Array Kit offers an antibody panel against a broad array of cytokines to enable measurement of their relative changes in cell culture supernatants.

Upon activation, naive CD4+ helper T cells differentiate into distinct functional subsets. The development of these subsets is driven, in part, by the cytokine milieu. Type 1 (Th1) cells help drive cellular immunity against intracellular pathogens. IL-12 and IFN-γ induce Th1 cell development. Th1 cells produce IFN-γ and IL-2, which provide a positive feedback loop to enhance Th1 cell differentiation and NK cell and CD8+ T cell cytolytic activity.

Th2 cells play a crucial role in the humoral immune response against extracellular pathogens. IL-4 drives development of Th2 cells, which subsequently produce IL-4, IL-5, and IL-13. These cytokines induce B cell proliferation, antibody production, IgE class switching, and activate eosinophils respectively.

Another distinct helper T cell lineage, Th17, is important for mucosal immunity. Dysregulation of Th17 may significantly contribute to the development of autoimmunity. IL-17 produced by Th17 cells induce secretion of pro-inflammatory cytokines IL-6, IL-8, GM-CSF, and TNF-α. Many of these molecules link innate and adaptive immunity through the recruitment and activation of innate immune cells.

Effective immune responses require finely tuned coordination between pro and anti-inflammatory signals. Pro-inflammatory molecules play important roles in activating key immune players to fight infection. IL-8 induces granulocyte migration and activates neutrophil phagocytic activity. GM-CSF mobilizes monocytes into infected tissue and activates macrophage and neutrophils. TNF-α is a multifunctional pro-inflammatory cytokine involved with a number of processes including cell proliferation, differentiation, and apoptosis.

Uncontrolled inflammation may damage surrounding host tissue. IL-10 is a prototypical anti-inflammatory cytokine that serves to terminate the acute inflammatory response by inhibiting Th1 cell function and pro-inflammatory cytokine production.

注意事项：

*Kit should be stored at 4°C.