PFKFB3 (D7H4Q) Rabbit mAb

• 产品编号：CST-13123S      品牌：CST       原厂货号：13123S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PFKFB3 (D7H4Q) Rabbit mAb兔单抗可以识别内源性PFKFB3总蛋白水平。单克隆抗体由合成肽段免疫动物产生，该肽段与人PFKFB3蛋白Leu456附近氨基酸一致。

双功能6-磷酸果糖激酶2/果糖2,6 -二磷酸酶2（PFK-2/FBPase或PFKFB）催化2,6-二磷酸果糖的合成和降解，并调节其稳态水平（1,2）。 2,6-二磷酸果糖激活磷酸果糖激酶，该酶是糖酵解的限速酶，通过变构调节作用调节糖酵解（1,2）。已经确定四种不同的PFKFB的亚型（PFKFB1，PFKFB2 PFKFB3，PFKFB4）（1,2）。其中，PFKFB3/iPFK-2被证明在缺氧条件下能被缺氧诱导表达，导致糖酵解增加（2）。研究表明，PFKFB3/iPFK-2也高表达于某些类型的人类癌症中（3）。

1. Atsumi, T. et al. (2005) Diabetes 54, 3349-57.
2. Kim, S.G. et al. (2006) J Biol Chem 281, 2939-44.
3. Atsumi, T. et al. (2002) Cancer Res 62, 5881-7.

原厂资料：

Specificity / Sensitivity

PFKFB3 (D7H4Q) Rabbit mAb recognizes endogenous levels of total PFKFB3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu456 of human PFKFB3 protein.

Background

The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). One of them, PFKFB3/iPFK-2, was shown to be inducible by hypoxia leading to increased glycolysis under hypoxic conditions (2). Research studies have shown that PFKFB3/iPFK-2 is also highly expressed in some types of human cancer (3).

1. Atsumi, T. et al. (2005) Diabetes 54, 3349-57.
2. Kim, S.G. et al. (2006) J Biol Chem 281, 2939-44.
3. Atsumi, T. et al. (2002) Cancer Res 62, 5881-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody