p63-α (D2K8X) XP? Rabbit mAb

• 产品编号：CST-13109P      品牌：Cell Signaling Technology       原厂货号：13109P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rabbit mAb可以识别内源性p63-α总蛋白。该抗体可以检测全长异构体（TAp63-α）和截短的δNp63-α异构体，但是不能识别p63-β或 p63-γ isoform.Rabbit mAb可以识别内源性p63-α总蛋白。该抗体可以检测全长异构体（TAp63-α）和截短的δNp63-α异构体，但是不能识别p63-β或 p63-γ isoform.单克隆抗体由合成肽段免疫动物产生，该肽段与人p63-α蛋白的羧基端一致。p53抑癌蛋白在细胞对DNA损伤和其他基因组畸变过程中发挥了重要作用。激活p53将导致细胞周期停滞和DNA修复或凋亡（1）。除了p53，哺乳动物细胞还含有2种p53家族成员，p63和p73，它们与p53在结构和功能上都很类似（2）。p63可以诱导p53-响应基因和凋亡，p63突变很少会导致肿瘤（2）。研究人员经常能在肺，头部和颈部鳞癌细胞中检测到p63基因的增殖（2,3）。p63基因含有一个选择性的转录起始位点会产生一个缺失转录激活结构域的40kDa δNp63，羧基端的选择性剪切会产生α, β, 和γ三种异构体（3,4）。

1. Levine, A.J. (1997) Cell 88, 323-31.
2. Waltermann, A. et al. (2003) Oncogene 22, 5686-93.
3. Hibi, K. et al. (2000) Proc Natl Acad Sci U S A 97, 5462-7.
4. Yang, A. et al. (1999) Nature 398, 714-8.

原厂资料：

Specificity / Sensitivity

p63-α (D2K8X) XP^® Rabbit mAb recognizes endogenous levels of total p63-α protein. This antibody will detect both the full-length isoform (TAp63-α) and the truncated δNp63-α isoform, but will not recognize the p63-β or p63-γ isoform.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p63-α protein.

Background

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2). While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2). Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck (2,3). The p63 gene contains an alternative transcription initiation site that yields a 40 kDa δNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms (3,4).

1. Levine, A.J. (1997) Cell 88, 323-31.
2. Waltermann, A. et al. (2003) Oncogene 22, 5686-93.
3. Hibi, K. et al. (2000) Proc Natl Acad Sci U S A 97, 5462-7.
4. Yang, A. et al. (1999) Nature 398, 714-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.