SCAP Antibody

• 产品编号：CST-13102S      品牌：CST       原厂货号：13102S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SCAP Antibody可以识别内源性SCAP总蛋白水平。多抗由合成肽段免疫动物产生，该肽段与人SCAP 蛋白Gly193邻近氨基酸序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。SREBP裂解活化蛋白(SCAP)是一种膜结合蛋白，在调节固醇调控元件结合蛋白(SREBP)处理过程中是必需的(1)。在胆固醇饥饿细胞中，SREBPs在SCAP的帮助下从内质网移动到高尔基体，此处前SREBPs被site-1 (S1P)和site-2 (S2P)蛋白酶裂解(1,2)。释放的SREBP氨基端结构域进入细胞核和负责胆固醇合成基因的启动子上的固醇应答元件结合(1,2)。研究表明，SCAP/ SREBP途径是小鼠糖尿病性脂肪肝发展和糖类诱导的高甘油三酯血症的关键（3）。此外，SCAP/ SREBP途径还能保护癌细胞免受脂毒性(4)。

1. Brown, M.S. and Goldstein, J.L. (1997) Cell 89, 331-40.
2. Horton, J.D. et al. (2002) J Clin Invest 109, 1125-31.
3. Moon, Y.A. et al. (2012) Cell Metab 15, 240-6.
4. Williams, K.J. et al. (2013) Cancer Res 73, 2850-62.

原厂资料：

Specificity / Sensitivity

SCAP Antibody recognizes endogenous levels of total SCAP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly193 of human SCAP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

SREBP cleavage activating protein (SCAP) is a membrane-bound protein essential in regulating sterol regulatory element binding protein (SREBP) processing (1). In cholesterol-starved cells, SREBPs move from the ER to the Golgi apparatus with the help of SCAP, where the precursor SREBPs are cleaved by site-1 (S1P) and site-2 (S2P) proteases (1,2). Released SREBP amino-terminal domains enter the nucleus and bind to sterol response elements located in the promoters of a number of genes responsible for the cholesterol synthesis (1,2). Research studies show that the SCAP/SREBP pathway is critical for diabetic fatty liver development and carbohydrate-induced hypertriglyceridemia in mice (3). In addition, the SCAP/SREBP pathway is required to protect cancer cells from lipotoxicity (4).

1. Brown, M.S. and Goldstein, J.L. (1997) Cell 89, 331-40.
2. Horton, J.D. et al. (2002) J Clin Invest 109, 1125-31.
3. Moon, Y.A. et al. (2012) Cell Metab 15, 240-6.
4. Williams, K.J. et al. (2013) Cancer Res 73, 2850-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.