PEA-15 (D1G6A) Rabbit mAb

• 产品编号：CST-13091S      品牌：CST       原厂货号：13091S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PEA-15 (D1G6A) Rabbit mAb可以识别内源性的PEA-15总蛋白该单克隆抗体采用合成的人PEA-15蛋白Leu60周围残疾相对应的肽段免疫动物产制备的。PEA-15是一个15 kDa的磷酸化蛋白，在星形胶质细胞、成纤维细胞以及组织（包括肺和眼）中表达（1）。研究证明，该蛋白协助调控细胞的生长、死亡，及葡萄糖利用（2-4）。PEA-15的氨基末端DED结构域介导它与FADD或Erk的结合，并进一步调控Erk和细胞凋亡信号通路。PEA-15可以在羧基末端的Ser104和Ser116两个丝氨酸残基处被磷酸化。在这些位点上的磷酸化调节其与Erk和FADD的结合（2,3）。

1. Danziger, N. et al. (1995) J. Neurochem. 64, 1016-1025.
2. Krueger, J. et al. (2005) Mol. Biol. Cell 16, 3552-3561.
3. Renganathan, H. et al. (2005) Biochem. J. 390, 729-735.
4. Condorelli, G. et al. (1998) EMBO J. 17, 3858-3866.

原厂资料：

Specificity / Sensitivity

PEA-15 (D1G6A) Rabbit mAb recognizes endogenous levels of total PEA-15 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu60 of human PEA-15 protein.

Background

PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as in tissues, including the lung and eye (1). The protein has been shown to coordinate cell growth, death, and glucose utilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and further regulates the Erk and apoptosis signaling pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3).

1. Danziger, N. et al. (1995) J. Neurochem. 64, 1016-1025.
2. Krueger, J. et al. (2005) Mol. Biol. Cell 16, 3552-3561.
3. Renganathan, H. et al. (2005) Biochem. J. 390, 729-735.
4. Condorelli, G. et al. (1998) EMBO J. 17, 3858-3866.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody