Troponin I (D6F8) Rabbit mAb

• 产品编号：CST-13083S      品牌：CST       原厂货号：13083S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Troponin I (D6F8) Rabbit mAb可以识别内源性总的troponin I蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人troponin I蛋白氨基端附近氨基酸一致。Troponin，与tropomyosin结合，作为一个分子开关调控细胞内Ca²⁺浓度改变引起的肌肉收缩。Troponin有三个亚基：Ca²⁺结合亚基troponin C(TnC)，tropomyosin结合亚基-troponin T（TnT）以及已知亚基troponin I (TnI) (1). β-肾上腺素刺激心脏后，TnI（心脏）的Ser23和Ser24会被PKA和PKC磷酸化。该磷酸化激发调控亚基TnC的结构变化，减少TnI 和TnC的相互作用，通过下降TnC与Ca²⁺ 的亲和性降低肌丝对Ca²⁺的敏感（1-3）。

1. Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
2. Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
3. Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Troponin I (D6F8) Rabbit mAb recognizes endogenous levels of total troponin I protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human troponin I protein.

Background

Troponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca²⁺ concentration. Troponin consists of three subunits: the Ca²⁺-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca²⁺ sensitivity by reducing the Ca²⁺ binding affinity of TnC (1-3).

1. Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
2. Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
3. Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody