Ring1A (D2P4D) Rabbit mAb

• 产品编号：CST-13069S      品牌：CST       原厂货号：13069S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Ring1A (D2P4D) Rabbit mAb兔单抗能够检测内源性 Ring1A总蛋白水平。该抗体不与Ring1B产生交叉反应。该单克隆抗体是采用合成的与人源Ring1A蛋白 Pro316残基周围序列相对应的肽段免疫动物而生产的。Ring1A在Polycomb-group蛋白质功能中起着一定作用。Polycomb-group (PcG) 蛋白形成两个明显的复合物，它们在发育时期参与Hox基因的转录抑制(1,2)。这些复合物是(a) EED-EZH2和(b) the PRC复合物，这是至少由Bmi-1和Ring1A/Ring1B组成。EZH2包含的复合物对H3K27的甲基化有作用，并且PRC复合物使H2A泛素化。EZH2甲基化在PRC泛素化之前被需要，并且两者都对Hox基因抑制有本质作用(3)。最近显示PcG蛋白可沉默一群发育重要的调节基因，被称为双价基因(4)。这个调节作用对于干细胞的自我更新能力或分化进入多潜能祖细胞(multipotent progenitors，MPPs )有作用。PcG蛋白促使的异常表观基因沉默也是被认为在肿瘤的发生上有着重要作用 (5)。

1. Cao, R. et al. (2005) Mol Cell 20, 845-54.
2. Otte, A.P. and Kwaks, T.H. (2003) Curr Opin Genet Dev 13, 448-54.
3. Stock, J.K. et al. (2007) Nat Cell Biol 9, 1428-35.
4. Sparmann, A. and van Lohuizen, M. (2006) Nat Rev Cancer 6, 846-56.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Guinea Pig

Specificity / Sensitivity

Ring1A (D2P4D) Rabbit mAb recognizes endogenous levels of total Ring1A protein. This antibody does not cross-react with Ring1B.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro316 of human Ring1A protein.

Background

Ring1A plays a role in polycomb group (PcG) protein function. PcG proteins are critically involved in transcriptional repression of Hox genes during development (1,2). PcG proteins form two distinct complexes: EED-EZH2 and the PRC complex, which is composed of at least Bmi1 and Ring1A/Ring1B. The EZH2-containing complex is responsible for the methylation of H3K27, and the PRC complex ubiquitylates H2A. EZH2 methylation is required prior to PRC ubiquitylation, and both are essential for Hox gene repression (3). It has recently been shown that PcG proteins silence a group of developmentally important regulator genes, referred to as bivalent genes (4). This regulation may be responsible for the ability of stem cells to self renew or switch to differentiate into multipotent progenitors. Aberrant epigenetic silencing by PcG proteins is also thought to be important in tumorigenesis (5).

1. Cao, R. et al. (2005) Mol Cell 20, 845-54.
2. Otte, A.P. and Kwaks, T.H. (2003) Curr Opin Genet Dev 13, 448-54.
3. Stock, J.K. et al. (2007) Nat Cell Biol 9, 1428-35.
4. Sparmann, A. and van Lohuizen, M. (2006) Nat Rev Cancer 6, 846-56.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.