Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb

• 产品编号：CST-13064S      品牌：CST       原厂货号：13064S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb兔单抗可以识别Ser483磷酸化的内源性PFKFB2总蛋白水平。单克隆抗体由合成的磷酸肽段免疫动物产生，该肽段与人PFKFB2蛋白Ser483 邻近氨基酸序列一致。双功能6-磷酸果糖激酶2/果糖2,6 -二磷酸酶2（PFK-2/FBPase或PFKFB）催化2,6-二磷酸果糖的合成和降解，并调节其稳态水平（1,2）。 2,6-二磷酸果糖激活磷酸果糖激酶，该酶是糖酵解的限速酶，通过变构调节作用调节糖酵解（1,2）。已经确定四种不同的PFKFB的亚型（PFKFB1，PFKFB2 PFKFB3，PFKFB4）（1,2）。其中，PFKFB3/iPFK-2被证明在缺氧条件下能被缺氧诱导表达，导致糖酵解增加（2）。研究表明，PFKFB3/iPFK-2也高表达于某些类型的人类癌症中（3）。

1. Atsumi, T. et al. (2005) Diabetes 54, 3349-57.
2. Kim, S.G. et al. (2006) J Biol Chem 281, 2939-44.
3. Novellasdemunt, L. et al. (2013) J Biol Chem 288, 10640-51.
4. Moon, J.S. et al. (2011) Biochem J 433, 225-33.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-PFKFB2 (Ser483) (D4R1W) Rabbit mAb recognizes endogenous levels of PFKFB2 protein only when phosphorylated at Ser483.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser483 of human PFKFB2 protein.

Background

The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). Research studies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2 (3). In addition, androgen increases the expression of PFKFB2 in prostate cancer cells (4).

1. Atsumi, T. et al. (2005) Diabetes 54, 3349-57.
2. Kim, S.G. et al. (2006) J Biol Chem 281, 2939-44.
3. Novellasdemunt, L. et al. (2013) J Biol Chem 288, 10640-51.
4. Moon, J.S. et al. (2011) Biochem J 433, 225-33.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.