Src (36D10) Rabbit mAb?(Pacific Blue? Conjugate)

• 产品编号：CST-13040S      品牌：Cell Signaling Technology       原厂货号：13040S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Src (36D10) Rabbit mAb (Pacific Blue™ Conjugate)能够检测内源性的Src蛋白。该抗体与其他的Src家族成员可能有交叉反应，但是与其他非家族成员蛋白没有交叉反应。该单克隆抗体是采用合成的与人源Src蛋白羧基端周围序列相一致的肽段免疫动物而产生的。此抗体偶联Pacific Blue™荧光素染料，可用于人类细胞样本的流式细胞分析检测。该抗体预期和未偶联的Src (36D10) Rabbit mAb #2109有相同的物种交叉反应性。Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的，但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化，能够上调酶活性；而蛋白羧基端尾部Tyr527位点被Csk磷酸化后，则会使酶的活性降低(2)。

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken

Specificity / Sensitivity

Src (36D10) Rabbit mAb (Pacific Blue™ Conjugate) detects endogenous levels of Src proteins. The antibody may cross-react with other Src family members, but does not cross-react with other non-family proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Src.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody Src (36D10) Rabbit mAb #2109.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the anti-
body. Protect from light. Do not freeze.