LKB1 (D60C5F10) Rabbit mAb (IHC Formulated)

• 产品编号：CST-13031S      品牌：CST       原厂货号：13031S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LKB1 (D60C5F10) Rabbit mAb 兔单抗(IHC Formulated)可以识别内源性LKB1总蛋白水平。单克隆抗体由重组的磷酸肽段免疫动物产生，该肽段与人LKB1蛋白羧基端邻近氨基酸序列一致。LKB1（STK11）是一个丝氨酸/苏氨酸激酶，也是一个肿瘤抑制因子，可以通过对多种下游激酶的调控而控制细胞结构、凋亡和能量平衡（1,2）。LKB1、一种推测激酶STRAD和MO25脚手架蛋白组成胞质蛋白复合物，它可以激活AMP-激活的蛋白激酶（AMPK）和几种AMPK-相关激酶（3）。AMPK是细胞能量平衡的主要调节者，控制下游效应器，从而调节对ATP浓度反应的细胞生长、凋亡（4）。LKB1似乎可以在细胞内在多个位点被磷酸化，包括人LKB1的Ser31/325/428和Thr189/336/363（5）。LKB1基因的相应变异会导致Peutz-Jeghers综合征（PJS），这是一宗常染色体显性的疾病，以良性胃肠道息肉和口、手、足处皮肤深色变为特点（6）。LKB1的其它一些突变与若干组织中的偶发癌症的形成有关（7）。

1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.

原厂资料：

Specificity / Sensitivity

LKB1 (D60C5F10) Rabbit mAb (IHC Formulated) recognizes endogenous levels of total LKB1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human LKB1 protein.

Background

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).

Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).

1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.