Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP??Rabbit mAb?(Biotinylated)

• 产品编号：CST-13020S      品牌：CST       原厂货号：13020S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗 (生物素标记的) 能够检测仅在 Ser240和Ser244位点磷酸化的内源性核糖体蛋白S6蛋白水平。该单克隆抗体是经由合成的围绕人核糖体蛋白S6的 Ser240 和 Ser244位点周围序列的磷酸化肽段免疫动物而生产的。CST抗体在最佳条件下偶联了生物素。并通过直标法流式细胞术在人细胞内进行内部检测。它与未接合的 Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb兔单抗 #5364具有相同的种属交叉反应性。生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关，而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5'TOP) 能够编码涉及细胞周期进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Pig

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP^® Rabbit mAb (Biotinylated) detects endogenous levels of ribosomal protein S6 only when phosphorylated at Ser240 and Ser244.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser240 and Ser244 of human ribosomal protein S6.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibodies