FAK (D2R2E) Rabbit mAb

• 产品编号：CST-13009S      品牌：CST       原厂货号：13009S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FAK (D2R2E) Rabbit mAb可以识别内源性总的FAK蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人FAK蛋白Ser893 附近氨基酸一致。黏着斑激酶(FAK)是一种广泛表达的细胞中蛋白酪氨酸激酶，参与整合素接到的信号转导。它在多种生物学过程，包括细胞扩散，迁移和存活的控制中起重要作用(1)。整合素激活FAK导致Tyr397自身磷酸化，这是一个结合Src家族激酶PI3K和PLCγ的位点(2-5)。募集Src家族激酶会使催化结构域的Tyr407, Tyr576,和 Tyr577磷酸化以及FAK羧基端的Tyr871和Tyr925磷酸化(6,7)。

1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

原厂资料：

Specificity / Sensitivity

FAK (D2R2E) Rabbit mAb recognizes endogenous levels of total FAK protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser893 of human FAK protein.

Background

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody