Phospho-YAP (Ser127) (D9W2I) Rabbit mAb

• 产品编号：CST-13008S      品牌：CST       原厂货号：13008S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-YAP (Ser127) (D9W2I) Rabbit mAb兔单抗特异性识别Ser127磷酸化的内源性YAP蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人YAP蛋白的Ser127邻近氨基酸残基序列一致。YAP (Yes-associated protein, YAP65)是基于它能够与Yes的SH3结构域结合的能力而被发现的。它也能和其他含有SH3结构域的蛋白结合，例如Nck，Crk，Src和Abl（1）。除了SH3结合基序，YAP含有一个PDZ相互作用基序，一个卷曲螺旋结构域和WW结构域（2-4）。对YAP的早期研究都指出它能够锚定并靶向特殊的亚细胞结构，之后的研究显示YAP是一个转录共激活因子，能够通过它的WW结构域和PEBP2以及其他转录因子的PY基序（PPxY）结合（5,6）。Ser127被磷酸化后，YAP通过Akt依赖的模式与14-3-3结合，抑制p73介导的凋亡（6）。

1. Sudol, M. (1994) Oncogene 9, 2145-52.
2. Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
3. Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
4. Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
5. Yagi, R. et al. (1999) EMBO J 18, 2551-62.
6. Basu, S. et al. (2003) Mol Cell 11, 11-23.
7. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
8. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
9. Yu, F.X. et al. (2012) Cell 150, 780-91.

原厂资料：

Specificity / Sensitivity

Phospho-YAP (Ser127) (D9W2I) Rabbit mAb recognizes endogenous levels of YAP protein only when phosphorylated at Ser127.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP protein.

Background

YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6).

1. Sudol, M. (1994) Oncogene 9, 2145-52.
2. Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
3. Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
4. Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
5. Yagi, R. et al. (1999) EMBO J 18, 2551-62.
6. Basu, S. et al. (2003) Mol Cell 11, 11-23.
7. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
8. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
9. Yu, F.X. et al. (2012) Cell 150, 780-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody