Myosin Light Chain 2v (D5I1C) (Cardiac Isoform) Rabbit mAb (Rodent Specific)

• 产品编号：CST-12975S      品牌：CST       原厂货号：12975S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

</p>Myosin Light Chain 2v (D5I1C) (心脏亚型) Rabbit mAb (啮齿类特异)可以识别内源性总的肌球蛋白轻链2v（心脏亚型）蛋白。</p>单克隆抗体由合成肽段免疫动物产生，该肽段与小鼠肌球蛋白轻链2v（心脏亚型）蛋白氨基末端附近氨基酸一致。</p>肌球蛋白由6条多肽链构成：两条相同的重链和两组轻链。肌球蛋白轻链2（MLC2），也被称为肌球蛋白调节轻链（MRLC），RLC或LC20，有许多取决于其分布的不同亚型。在平滑肌中，MLC2会被肌球蛋白轻链激酶（MLCK）在Ca2+/calmodulin依赖的状态下磷酸化第18位苏氨酸和19位丝氨酸（1）。这种磷酸化与肌球蛋白ATPase活性和平滑肌收缩性相关（2）。ROCK也会磷酸化平滑肌MLC2的19位丝氨酸，这会调节应力纤维的集合（3）。心脏中表达两种类型的肌球蛋白轻链，肌球蛋白轻链2v(MYL2, MLC-2v)表达限制在心室中，肌球蛋白轻链2a(MYL7, MLC-2a)的特异性表达在心房中。中左心房过厚引起的心肌肥大病人发现他们的MYL2基因发生了突变。

1. Ikebe, M. and Hartshorne, D.J. (1985) J Biol Chem 260, 10027-31.
2. Tan, J.L. et al. (1992) Annu Rev Biochem 61, 721-59.
3. Tsukamoto, O. and Kitakaze, M. (2013) Circ J 77, 2218-25.

原厂资料：

Specificity / Sensitivity

<p>Myosin Light Chain 2v (D5I1C) (Cardiac Isoform) Rabbit mAb (Rodent Specific) recognizes endogenous levels of total myosin light chain 2v (cardiac isoform) protein.

Source / Purification

<p>Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse myosin light chain 2v (cardiac isoform) protein.

Background

<p>Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms with varying tissue distribution. Smooth muscle MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca²⁺/calmodulin-dependent manner (1). These phosphorylation events are correlated with myosin ATPase activity and smooth muscle contraction (2). Striated muscle contraction is regulated by the troponin-tropomyosin complex in thin actin filaments and by binding of Ca²⁺ to troponin C (3). Two types of myosin light chain are expressed in the heart, with myosin light chain 2v (MYL2, MLC-2v) expression restricted to the ventricles and myosin light chain 2a (MYL7, MLC-2a) found specific to the atria. Mutations in the corresponding <i>MYL2</i> gene are found in patients diagnosed with a form of hypertrophic cardiomyopathy characterized by thickening of the mid-left ventricle.

1. Ikebe, M. and Hartshorne, D.J. (1985) J Biol Chem 260, 10027-31.
2. Tan, J.L. et al. (1992) Annu Rev Biochem 61, 721-59.
3. Tsukamoto, O. and Kitakaze, M. (2013) Circ J 77, 2218-25.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody