Color-coded Prestained Protein Marker High Range (43-315 kDa)

• 产品编号：CST-12949P      品牌：CST       原厂货号：12949P
• 产品分类：蛋白质检测与分析 > 蛋白质凝胶电泳 > 蛋白分子量标准品 > 预染蛋白标准品
• 应用分类：

描述：

Color-coded Prestained Protein Marker，大范围(43 - 315 kDa)是纯化蛋白的混合物,蓝色,绿色，橙色或粉色复合8条带横跨43至315 kDa。蛋白质浓度要仔细定量。染料结合到蛋白质会影响在SDS-PAGE凝胶电泳中的移动。蛋白质的分子量如右图所示。保存：在62.5 mM Tris-H₃PO₄ (pH 7.5 at 25ºC), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ 和33% glycerol溶液中保存。储存于–20°C可保存一年。不煮蛋白标记。1.在室温下解冻蛋白。2.轻轻地涡旋溶液,以确保混合均匀。3.每条道上样适量的蛋白标记,如以下规定:-Mini-Gel: 0.75 -1.0毫米厚:上样10μl 1.5毫米厚:上样20μl 大的凝胶:0.75 -1.0毫米厚:上样20μl 1.5毫米厚:上样40μl 4.未使用的蛋白储存于-20ºC以利长期保存。

原厂资料：

Description

Color-coded Prestained Protein Marker, High Range (43-315 kDa) is a mixture of purified proteins, covalently coupled to blue, green, orange, or pink dyes, that resolves to 8 bands between 43 and 315 kDa when electrophoresed. The protein concentrations are carefully balanced for even intensity. The covalent coupling of dye to protein affects the electrophoretic mobility in SDS-PAGE gels relative to uncoupled proteins. The apparent molecular weights of the prestained proteins are shown in the image to the right.

Bioactivity

Storage: Supplied in 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25ºC), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol. Store at –20°C for up to one year.

Directions for Use

Important: Do Not Boil Protein Marker

1. Thaw the protein ladder at room temperature.

2. Gently vortex solution to ensure the mixture is homogeneous.

3. Load the appropriate volume of the protein marker per lane, as specified below:

-Mini-Gel:

0.75-1.0 mm thick: load 10 μl

1.5 mm thick: load 20 μl

-Large Gel:

0.75-1.0 mm thick: load 20 μl

1.5 mm thick: load 40 μl

4. Unused ladder may be returned to -20ºC for long-term storage.

注意事项：

Storage: Supplied in 62.5 mM Tris-H3
PO4 (pH 7.5 at 25ºC),
1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3
 and 33%
glycerol. Store at –20°C for up to one year.