SYVN1 Antibody

• 产品编号：CST-12925S      品牌：Cell Signaling Technology       原厂货号：12925S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SYVN1抗体识别内源性的synoviolin-1总蛋白。此抗体不与AMFR/gp78发生交叉反应。此多克隆抗体由人synoviolin-1蛋白羰基末端的邻近氨基酸残基序列一致的肽段，免疫动物产生。抗体经Protein A和肽亲和层析纯化。Synoviolin-1(SYVN1 / HRD1)是一个环形E3泛素蛋白连接酶和内质网(ER)质量控制系统的主要组件，参与错误折叠蛋白泛素依赖性的降解 (1)。SYVN1 是一个多次跨膜的ER蛋白，在ER应激条件下，其蛋白表达量上调(1 - 4)。研究表明SYVN1是一种与关节病的发病机制相关的抗凋亡因子，促进滑膜增生(5)。此外,基因靶向研究已经证明,SYVN1表达式是胚胎发生中必不可少(6)。

1. Kikkert, M. et al. (2004) J Biol Chem 279, 3525-34.
2. Kaneko, M. et al. (2007) FEBS Lett 581, 5355-60.
3. Yamamoto, K. et al. (2008) J Biochem 144, 477-86.
4. Nadav, E. et al. (2003) Biochem Biophys Res Commun 303, 91-7.
5. Amano, T. et al. (2003) Genes Dev 17, 2436-49.
6. Yagishita, N. et al. (2005) J Biol Chem 280, 7909-16.

原厂资料：

Specificity / Sensitivity

SYVN1 Antibody recognizes endogenous levels of total synoviolin-1 protein. This antibody does not cross-react with AMFR/gp78.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human synoviolin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Synoviolin-1 (SYVN1/HRD1) is a RING-type E3 ubiquitin-protein ligase and major component of the endoplasmic reticulum (ER) quality control system that is involved in the ubiquitin-dependent degradation of misfolded proteins (1). SYVN1 is a multispanning ER membrane protein whose expression is upregulated at the protein level under conditions that promote ER stress (1-4). Research studies have shown that SYVN1 is an anti-apoptotic factor that is implicated in the pathogenesis of arthropathy by promoting synovial hyperplasia (5). Furthermore, gene-targeting studies have demonstrated that SYVN1 expression is indispensable for embryogenesis (6).

1. Kikkert, M. et al. (2004) J Biol Chem 279, 3525-34.
2. Kaneko, M. et al. (2007) FEBS Lett 581, 5355-60.
3. Yamamoto, K. et al. (2008) J Biochem 144, 477-86.
4. Nadav, E. et al. (2003) Biochem Biophys Res Commun 303, 91-7.
5. Amano, T. et al. (2003) Genes Dev 17, 2436-49.
6. Yagishita, N. et al. (2005) J Biol Chem 280, 7909-16.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.