CBFβ Antibody

• 产品编号：CST-12902S      品牌：Cell Signaling Technology       原厂货号：12902S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

CBFβ Antibody recognizes endogenous levels of total CBFβ protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro36 of human CBFβ protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

CBFβ Antibody recognizes endogenous levels of total CBFβ protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro36 of human CBFβ protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Core-binding factor subunit β (CBFβ) is a non-DNA binding transcription factor subunit that associates with and regulates the DNA binding activity of RUNX1, RUNX2, and RUNX3 (1). CBFβ is ubiquitously expressed and has been implicated in a variety of developmental processes including hematapoiesis, T cell development, chondrogenesis, and bone formation (2-7). In addition, investigators have identified CBFβ as one of the most frequently translocated genes in leukemia (8) and research studies have found it to be required for HIV immune evasion (9,10). CBFβ interacts with the viral protein VIF and triggers assembly of a ubiquitin ligase complex that targets the retroviral inhibitor APOBEC3G for degradation (9,10).

1. Ogawa, E. et al. (1993) Virology 194, 314-31.
2. Wang, Q. et al. (1996) Cell 87, 697-708.
3. Talebian, L. et al. (2007) Blood 109, 11-21.
4. Johnson, K. et al. (2012) Science 336, 717-21.
5. Yoshida, C.A. et al. (2002) Nat Genet 32, 633-8.
6. Miller, J. et al. (2002) Nat Genet 32, 645-9.
7. Kundu, M. et al. (2002) Nat Genet 32, 639-44.
8. Goyama, S. and Mulloy, J.C. (2011) Int J Hematol 94, 126-33.
9. Jäger, S. et al. (2012) Nature 481, 371-5.
10. Zhang, W. et al. (2012) Nature 481, 376-9.

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.