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Human MCP-1

 
包装:
运保温度: 4ºC
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描述:

Source / Purification

 

Recombinant Human MCP-1 (hMCP-1) Gln24-Thr99 (Accession #NP_002973) was expressed in E.coli at Cell Signaling Technology.

 

Molecular Characterization

Molecular Characterization: Recombinant hMCP-1 has a calculated MW of 8,685. DTT-reduced protein migrates as a 10 kDa polypeptide. The nonreduced protein migrates at 12 kDa. The expected amino-terminal QPDAI of recombinant hMCP-1 was verified by amino acid sequencing.

Purity

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hMCP-1. All lots are greater than 95% pure.

Bioactivity

The activity of hMCP-1 was determined using a THP-1 cell migration assay. The ED50 of each lot is between 1-11 ng/ml.


注意事项:

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.


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