Human MCP-1

• 产品编号：CST-12900SC      品牌：CST       原厂货号：12900SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 趋化因子
• 应用分类：

描述：

Source / Purification

Recombinant Human MCP-1 (hMCP-1) Gln24-Thr99 (Accession #NP_002973) was expressed in E.coli at Cell Signaling Technology.

Molecular Characterization

Molecular Characterization: Recombinant hMCP-1 has a calculated MW of 8,685. DTT-reduced protein migrates as a 10 kDa polypeptide. The nonreduced protein migrates at 12 kDa. The expected amino-terminal QPDAI of recombinant hMCP-1 was verified by amino acid sequencing.

Purity

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hMCP-1. All lots are greater than 95% pure.

Bioactivity

The activity of hMCP-1 was determined using a THP-1 cell migration assay. The ED₅₀ of each lot is between 1-11 ng/ml.

原厂资料：

Molecular Characterization

Molecular Characterization: Recombinant hMCP-1 has a calculated MW of 8,685. DTT-reduced protein migrates as a 10 kDa polypeptide. The nonreduced protein migrates at 12 kDa. The expected amino-terminal QPDAI of recombinant hMCP-1 was verified by amino acid sequencing.

Purity

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hMCP-1. All lots are greater than 95% pure.

Bioactivity

The activity of hMCP-1 was determined using a THP-1 cell migration assay. The ED₅₀ of each lot is between 1-11 ng/ml.

Applications

Optimal concentration for the desired application should be determined by the user.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hMCP-1.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of hMCP-1 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hMCP-1. Carrier free: Lyophilized from a 0.22 μm filtered solution of hMCP-1 in 20 mM Tris, pH 7.2.

Background

MCP-1 (CCL2) is the first member of the C-C family of chemokines to be identified (1). C-C chemokines are characterized by two adjacent cysteine residues within the polypeptide, which form an intra-molecular disulfide bond. MCP-1 is a potent chemotactic factor for monocytes/macrophages, T cells and a subset of NK cells (1-4). The MCP-1 receptor, CCR2, is expressed as two splice isoforms, CCR2A and CCR2B, of which CCR2B is the predominant form (1). MCP-1 is secreted by adipocytes and appears to be one of many links between obesity, inflammation, and diabetes (1). MCP-1/CCR2 signaling appears to play a key role in γδ effector T cells recruitment and anti-tumor responses in a murine B16 melanoma model (2). Conversely, CCL2 expression is upregulated in many types of cancer and has been implicated in promoting tumor cell survival, proliferation, and tumor associated inflammation (4).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.