Phospho-Tau (Thr181) (D9F4G) Rabbit mAb

• 产品编号：CST-12885S      品牌：CST       原厂货号：12885S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tau (Thr181) (D9F4G) Rabbit mAb 兔单抗可以识别内源性的Thr181位点磷酸化Tau蛋白。该单克隆抗体由合成的人Tau蛋白Thr181 位点附近磷酸化肽段免疫动物产生。Tau是微管相关蛋白异构物，促进和稳定微管装配，尤其是在轴突中。有六个异构体已经被识别，它们有不同的氨基末端插入序列和不同数量的羧基末端串联重复序列，tau蛋白可被ERK、GSK-3和CDK5在大约25个位点处被过度磷酸化（1-2）。Tau蛋白磷酸化可降低其与微管的结合能力。神经纤维缠结是阿尔茨海默氏症的一个主要标志，这些缠结是由过度磷酸化tau蛋白组成的成对螺旋细丝束。GSK-3或CDK5对Tau蛋白Ser396的磷酸化可以导致微管的破坏，特别是在阿尔茨海默氏症中。此外，Tau蛋白包涵体还在其它神经退行性疾病中被发现，这些疾病被统称为Tau蛋白病(1,3)。研究已表明，阿尔茨海默氏病中，Tau在Thr181磷酸化是一个神经退行性疾病的标志(4)。

1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
4. Mitchell, A.J. (2009) J Neurol Neurosurg Psychiatry 80, 966-75.

原厂资料：

Specificity / Sensitivity

Phospho-Tau (Thr181) (D9F4G) Rabbit mAb recognizes endogenous levels of Tau protein only when phosphorylated at Thr181.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr181 of human Tau protein.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).The cerebrospinal fluid concentration of Tau phosphorylated at Thr181 has been proposed to be a biomarker for the study of neurodegenerative disorders (4).

1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
4. Mitchell, A.J. (2009) J Neurol Neurosurg Psychiatry 80, 966-75.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.