IRS-1 Inhibition Antibody Sampler Kit

• 产品编号：CST-12879S      品牌：Cell Signaling Technology       原厂货号：12879S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

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描述：

每个活化状态的抗体可以识别其各自靶点的磷酸化形式。所有的靶点残基都是基于小鼠的IRS-1序列的，除了Ser636/339和Ser1101，是基于人IRS-1的序列。IRS-1 (D23G12) Rabbit mAb可以识别总的IRS-1蛋白而不管其磷酸化状态。IRS-1 Inhibition Antibody Sampler试剂盒提供了经济的方式通过多种IRS-1丝氨酸残基的磷酸化状态检测胰岛素信号通路的负反馈过程。该试剂盒提供了足以进行4次western blot分析的一抗。胰岛素受体底物1(IRS-1)是胰岛素受体激酶的一类主要底物(1)。 IRS-1包含多个酪氨酸磷酸化位点，可以作为含SH2结构域蛋白的停靠位点，这些蛋白调控着胰岛素的代谢和促生长功能(2-4)。IRS-1还含有超过30个潜在的丝氨酸/苏氨酸磷酸化位点，其中多个和胰岛素信号通路中的负反馈机制项链。IRS-1的Ser302(human Ser307)可以被FOX01 (5), IKKγ, 和 MYO1C (6)调控。IRS-1的Ser307(human Ser312)被JNK (7)和 IKK (8)磷酸化。胰岛素受体活化或其他刺激因素如TPA，IL-6和 视黄酸处理会引发PKC磷酸化小鼠IRS-1的Ser318(human Ser323) (9-12)。PKC和mTOR信号通路能够分别介导IRS-1的Ser612(human Ser616)和Ser632?635（human Ser636/639）磷酸化(13,14)。IRS-1的Ser1097 (human Ser1101)通过PKCθ介导磷酸化会导致细胞胰岛素通路的抑制，提示在某些肥胖模型中存在胰岛素抵抗的潜在机制(15)。

原厂资料：

Specificity / Sensitivity

Each activation state antibody recognizes the phosphorylated form of its target. All target residues are based on the sequence for mouse IRS-1, except Ser636/339 and Ser1101, which are based on the sequence for human IRS-1. IRS-1 (D23G12) Rabbit mAb recognizes total IRS-1 protein independent of its phosphorylation state.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding mouse Ser307 (human Ser312), human Ser636/639 (mouse Ser632/635), and human Ser1101 (mouse Ser1097) of IRS-1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding mouse Ser302 (human Ser307), mouse Ser318 (human Ser323), and mouse Ser612 (human 616) of IRS-1. IRS-1 (D23G12) rabbit monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Ser270 of human IRS-1 (mouse 1097).

Description

The IRS-1 Inhibition Antibody Sampler Kit provides an economical means to evaluate insulin signaling negative feedback loops via phosphorylation of various IRS-1 serine residues. The kit includes enough antibody to perform four western blot experiments with each primary antibody.

Background

Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites, many of which are related to negative feedback loops activated during insulin signaling. Ser302 (human Ser307) of IRS-1 is regulated by FOX01 (5), IKKγ, and MYO1C (6). Ser307 (human Ser312) of IRS-1 is phosphorylated by JNK (7) and IKK (8). PKC phosphorylates mouse IRS-1 at Ser318 (human Ser323) by insulin receptor activation or by other stimulation such as TPA, IL-6, and retinoic acid treatment (9-12). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 (human Ser616) and Ser632/635 (human Ser636/639), respectively (13,14). Phosphorylation of IRS-1 at Ser1097 (human Ser1101) is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (15).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.
Recommended Antibody Dilutions:
Western blotting  1:1000