SignalSlide??NF-κB p65?IHC Controls

• 产品编号：CST-12873S      品牌：CST       原厂货号：12873S
• 产品分类：生化和细胞分析 > 其它细胞分析试剂盒 > IHC 和 ICC 试剂盒
• 应用分类：

描述：

Description

Each control slide contains formalin fixed, paraffin-embedded HCT 116 cells, both untreated and treated with hTNF-α, that serve as a control for NF-κB p65 immunostaining.

原厂资料：

Description

Each control slide contains formalin fixed, paraffin-embedded HCT 116 cells, both untreated and treated with hTNF-α, that serve as a control for NF-κB p65 immunostaining.

Applications

These slides are intended for use in immunohistochemical assays.

Background

Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

注意事项：

Storage: Store at 4°C.
Optimal staining is achieved if slides are stained following CST’s
standard IHC protocols and are used within 8 weeks of assay
date; however, signals may persist beyond two months