Glycolysis II Antibody Sampler Kit

产品编号：CST-12866S 品牌：Cell Signaling Technology 原厂货号：12866S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1Kit

运保温度:

–20°C

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

其它组分：

PGAM1 (D3J9T) Rabbit mAb 【子货号：#12098，包装：40 µl，，运保温度： –20°C】

PFKFB2 (D7G5R) Rabbit mAb 【子货号：#13045，包装：40 µl，，运保温度： –20°C】

PFKFB3 (D7H4Q) Rabbit mAb 【子货号：#13123，包装：40 µl，，运保温度： –20°C】

Enolase-1 Antibody 【子货号：#3810，包装：40 µl，，运保温度： –20°C】

PDHK1 (C47H1) Rabbit mAb 【子货号：#3820，包装：40 µl，，运保温度： –20°C】

Anti-rabbit IgG, HRP-linked Antibody 【子货号：#7074，包装：100 µl，，运保温度： –20°C】

Aldolase A (D73H4) Rabbit mAb 【子货号：#8060，包装：40 µl，，运保温度： –20°C】

Enolase-2 (D20H2) Rabbit mAb 【子货号：#8171，包装：40 µl，，运保温度： –20°C】

PFKL Antibody 【子货号：#8175，包装：40 µl，，运保温度： –20°C】

描述：

分别合成与人果糖二磷酸醛缩酶A的Pro263邻近氨基酸残基序列，人烯醇化酶2蛋白的羧基端序列，人PFKFB2蛋白果糖2,6 -二磷酸酶区域的Pro454邻近氨基酸残基序列，人PFKFB3蛋白的Leu456邻近氨基酸残基序列，和人PGAM1羧基端序列一致的肽段免疫动物获得单抗。分别合成与人烯醇化酶1和人PFKL蛋白羧基端序列一致的肽段免疫动物获得多抗。多抗由蛋白A和肽亲和层析纯化。Glycolysis II Antibody Sampler 试剂盒提供了一种经济的方式研究参与糖酵解的一些酶。该试剂盒提供了足以进行4次western blot分析的一抗。糖酵解指的是葡萄糖在一系列酶催化反应下转变成丙酮酸的过程。磷酸果糖激酶(PFK)负责在糖酵解中将6-磷酸果糖进一步磷酸化(6)。双功能6-磷酸果糖激酶2/果糖2,6 -二磷酸酶2（PFK-2/FBPase或PFKFB）催化2,6-二磷酸果糖的合成和降解，并调节其稳态水平。已经发现了四种不同的PFKFB亚型(PFKFB1, PFKFB2, PFKFB3, 和 PFKFB4)。醛缩酶(果糖二磷酸醛缩酶)是一种催化果糖1,6-二磷酸为3-磷酸甘油的糖酵解酶(3)。磷酸甘油酸变位酶(PGAM1)在糖酵解过程中催化3-磷酸甘油酸转换为2-磷酸甘油酸(4)。烯醇化酶是一种重要的糖酵解酶参与2-磷酸甘油酸和磷酸烯醇式丙酮酸的互变。哺乳动物烯醇化酶有三种亚基: 烯醇化酶-1 (α-enolase), 烯醇化酶-2 (γ-enolase) 和烯醇化酶-3 ，可以形成同源和异源二聚体(5)。(β-enolase)丙酮酸脱氢酶激酶（PDHK）磷酸化PDH并失活它，而PDH的磷酸化是通过病痛酸脱氢酶磷酸酶介导的以产生活性的形式(6)。

原厂资料：

Specificity / Sensitivity

Each antibody recognizes endogenous total levels of the specified target protein independent of its modified state.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptides corresponding to residues surrounding Pro263 of human fructose bisphosphate aldolase A protein, the carboxy terminus of human enolase-2 protein, human PDHK1 protein, residues surrounding Pro454 within the fructose-2,6-biphosphatase region of human PFKFB2 protein, residues surrounding Leu456 of human PFKFB3 protein, and the carboxy terminus of human PGAM1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human enolase-1 and carboxy terminus of human PFKL protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level. Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (2). Aldolase (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde (3). Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis (4). Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers (5). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (6).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000