uPAR (D4Q5S) Rabbit mAb

• 产品编号：CST-12863S      品牌：CST       原厂货号：12863S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

uPAR (D4Q5S) Rabbit mAb可以识别内源性总的uPAR蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人uPAR蛋白Thr108附近氨基酸一致。人体尿激酶型纤溶酶原激活物受体（uPAR的）是一个55-65 kDa的，高度糖基化，GPI锚定的细胞表面受体（去糖基化蛋白质为35 kDa）(1-3)。它是纤溶酶原激活通路的核心成员。uPAR高亲和地与丝氨酸蛋白酶尿激酶型纤溶酶原激活剂(uPA)结合并在细胞表面上以空间限制的方式将纤溶酶原转化为活性形式的纤维蛋白溶酶(4)。纤维蛋白溶酶进一步激活uPA，这是由丝氨酸蛋白酶抑制剂所抑制的，例如纤溶酶原激活物抑制剂(5)。因此，uPAR在调控细胞外蛋白水解的过程中发挥了关键作用。此外，uPAR还调节了细胞增殖，黏附和运动(6,7)。研究表明多种癌细胞和组织中uPAR都是过表达的(8,9)。

1. Nielsen, L.S. et al. (1988) J Biol Chem 263, 2358-63.
2. Behrendt, N. et al. (1990) J Biol Chem 265, 6453-60.
3. Roldan, A.L. et al. (1990) EMBO J 9, 467-74.
4. Ellis, V. et al. (1991) J Biol Chem 266, 12752-8.
5. Ellis, V. et al. (1990) J Biol Chem 265, 9904-8.
6. Liu, D. et al. (2002) Cancer Cell 1, 445-57.
7. Waltz, D.A. et al. (1997) J Clin Invest 100, 58-67.
8. Blasi, F. and Sidenius, N. (2010) FEBS Lett 584, 1923-30.
9. Mazar, A.P. et al. (2011) Curr Pharm Des 17, 1970-8.

原厂资料：

Specificity / Sensitivity

uPAR (D4Q5S) Rabbit mAb recognizes endogenous levels of total uPAR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr108 of human uPAR protein.

Background

The human urokinase-type plasminogen activator receptor (uPAR) is a 55-65 kDa, highly glycosylated, GPI-anchored cell surface receptor (the deglycosylated protein is 35 kDa) (1-3). It is a central player in the plasminogen activation pathway. uPAR binds with high affinity to a serine protease urokinase-type plasminogen activator (uPA) and converts plasminogen to its active form plasmin in a spatially restricted manner on the cell surface (4). Plasmin further carries out the activation of uPA, which is inhibited by serpins, such as plasminogen activator inhibitors (5). Therefore, uPAR plays a key role in regulating extracellular proteolysis. In addition, uPAR plays an important role in regulating cell proliferation, adhesion and mobility (6,7). Research studies have shown that overexpression of uPAR is found in various cancer cells and tissues (8,9).

1. Nielsen, L.S. et al. (1988) J Biol Chem 263, 2358-63.
2. Behrendt, N. et al. (1990) J Biol Chem 265, 6453-60.
3. Roldan, A.L. et al. (1990) EMBO J 9, 467-74.
4. Ellis, V. et al. (1991) J Biol Chem 266, 12752-8.
5. Ellis, V. et al. (1990) J Biol Chem 265, 9904-8.
6. Liu, D. et al. (2002) Cancer Cell 1, 445-57.
7. Waltz, D.A. et al. (1997) J Clin Invest 100, 58-67.
8. Blasi, F. and Sidenius, N. (2010) FEBS Lett 584, 1923-30.
9. Mazar, A.P. et al. (2011) Curr Pharm Des 17, 1970-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.