Sin1 (D7G1A) Rabbit mAb

• 产品编号：CST-12860S      品牌：CST       原厂货号：12860S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Sin1 (D7G1A) Rabbit mAb兔单抗可以识别内源性总的Sin1蛋白，包括Sin1.1和Sin1.2亚型。单克隆抗体由合成肽段免疫动物产生，该肽段与人Sin1蛋白羧基端附近氨基酸一致。细胞生长是一个基本的生物过程，细胞积累的质量和大小的增加。哺乳动物TOR（mTOR）通路通过生长因子来源的信号协调能量和营养信号以调控生长（1）。mTOR是一个大的蛋白激酶，是两个不同复合物的组分。mTOR复合物（mTORC1），雷帕霉素的靶点，包括了mTOR, mTOR, GβL, Sin1,和 rictor (1). mTORC2复合物在体外能磷酸化Akt/PKB的Ser473（2）。这个磷酸化对总Akt/PKB的活化是必须的。不仅如此，siRNA敲除rictor后能够抑制在3T3-L1脂肪细胞中Ser473的磷酸化(3)。已经证明mTORC2可以磷酸化雷帕霉素耐受的S6K1突变体，这是另一个mTOR效应分子（4）。除此以外，Akt/PKB磷酸化Sin1的Thr86已经被证明可以在生长因子刺激后调控脂肪细胞中的mTORC2的活性（5）。

1. Sarbassov, D.D. et al. (2004) Curr Biol 14, 1296-302.
2. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
3. Hresko, R.C. and Mueckler, M. (2005) J Biol Chem 280, 40406-16.
4. Ali, S.M. and Sabatini, D.M. (2005) J Biol Chem 280, 19445-8.
5. Humphrey, S.J. et al. (2013) Cell Metab 17, 1009-20.

原厂资料：

Specificity / Sensitivity

Sin1 (D7G1A) Rabbit mAb recognizes endogenous levels of total Sin1 protein, including isoforms Sin1.1 and Sin1.2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Sin1 protein.

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase that is a component of two different complexes. The mTOR complex 1 (mTORC1), a target of rapamycin, contains mTOR, GβL, and raptor. mTORC2, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTORC2 complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). mTORC2 has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). In addition, phosphorylation of Sin1 at Thr86 by Akt/PKB was shown to regulate the activity of mTORC2 in adipocytes upon stimulation by growth factors (5).

1. Sarbassov, D.D. et al. (2004) Curr Biol 14, 1296-302.
2. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
3. Hresko, R.C. and Mueckler, M. (2005) J Biol Chem 280, 40406-16.
4. Ali, S.M. and Sabatini, D.M. (2005) J Biol Chem 280, 19445-8.
5. Humphrey, S.J. et al. (2013) Cell Metab 17, 1009-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.