SWI/SNF Complex Antibody Sampler Kit

• 产品编号：CST-12854S      品牌：Cell Signaling Technology       原厂货号：12854S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

试剂盒中的每种抗体都能够检测内源性特异目的蛋白的总水平，不会与其他家族成员发生交叉反应。ARID1A/BAF250A (D2A8U) Rabbit mAb还能与一个65 kDa的未知来源蛋白发生交叉反应。单克隆抗体经由合成的多肽免疫动物而生产的，对应序列信息如下：围绕人ARID1A/BAF250A的Gly1293残基，人BRM的Gly264残基，围绕人SMARCC1/BAF155蛋白的Gly975残基以及围绕人SMARCC2/BAF170的Ile818残基或人SNF5蛋白的Gln244残基。多克隆抗体是经由合成的围绕人Brg1的Ala52残基的多肽免疫动物而生产的。多克隆抗体由蛋白质A和多肽亲和层析纯化。

原厂资料：

Specificity / Sensitivity

Each antibody in this kit recognizes endogenous levels of total protein for the specified target and does not cross-react with other family members. ARID1A/BAF250A (D2A8U) Rabbit mAb also cross-reacts with proteins of unknown origin at 65 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1293 of human ARID1A/BAF250A protein, Gly264 of human BRM protein, Gly975 of human SMARCC1/BAF155 protein, Ile818 of human SMARCC2/BAF170 protein, or Gln244 of human SNF5 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala52 of human Brg1 protein. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.

Description

The SWI/SNF Complex Antibody Sampler Kit provides an economical means of detecting total protein from the SWI/SNF family members including ARID1A/BAF250A, Brg1, BRM, SMARCC1/BAF155, SMARCC2/BAF170 and SNF5. The kit contains enough primary antibody to perform four western blots per primary antibody.

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines (10-13). SMARCC1/BAF155, SMARCC2/BAF170, and SNF5 are members of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by BRG1 in vitro (14). ARID1A/BAF250A is one of the accessory subunits of the SWI/SNF complex (15). SMARCC1, SNF5, and ARID1A are an essential part of the mouse embryonic stem cell specific SWI/SNF complex (esBAF). SMARCC1 is necessary for early embryogenesis, especially proper brain and visceral endoderm development (16-18). SNF5 is necessary for early embryogenesis and hepatocyte differentiation (19,20). ARID1A is critical for ES cell pluripotency and differentiation into mesoderm-derived cardiomyocytes and adipocytes (15). While SMARCC2 has been shown to be part of the SWI/SNF complex in non-pluripotent cells, it is absent in pluripotent embryonic stem (ES) cells. Expression of SMARCC2 has been shown to be up-regulated in neurons/neuronal progenitors upon differentiation of mouse ES cells with retinoic acid, and exogenous expression of SMARCC2 leads to loss of stem cell pluripotency and self renewal (21).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibodies.
Recommended Antibody Dilutions:
Western blotting  1:1000