PathScan??Total Androgen Receptor Sandwich ELISA Kit

• 产品编号：CST-12850C      品牌：CST       原厂货号：12850C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

其它组分：

描述：

.PathScan® Total Androgen Receptor Sandwich ELISA Kit能够识别内源性水平的雄激素受体蛋白，见图1.该试剂盒的灵敏度见图2.本试剂盒用来检测指示物种的蛋白，即通过内部测试确定的那些，也可以用来检测来自其他物种的同源蛋白。PathScan® Total Androgen Receptor Sandwich ELISA Kit是一个固相夹心酶联免疫吸附试验（ELISA），能够检测内源性水平的总雄激素受体蛋白。微孔已经包被Androgen Receptor Rabbit mAb。细胞裂解物孵育后，雄激素受体蛋白由包被的抗体捕获。充分洗涤后，加入Androgen Receptor Mouse Detection mAb检测捕获的雄激素受体蛋白。然后用抗小鼠IgG，HRP标记的抗体识别结合的检测抗体。加入HRP底物TMB进行显色。显色吸光度的大小与总雄激素受体蛋白量成正比。该试剂盒中的抗体是针对试剂盒定制的。雄激素受体（AR）是锌指转录因子，属于核受体超家族，通过磷酸化和配体结合的二聚化被激活（1）。这促进了细胞核定位和雄激素靶基因中雄激素与应答元件的结合。 AR在男性发育和前列腺癌发展的几个阶段起着至关重要的作用（2,3）。

1. Li, J. and Al-Azzawi, F. (2009) Maturitas 63, 142-8.
2. Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
3. Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.

原厂资料：

Specificity / Sensitivity

PathScan® Total Androgen Receptor Sandwich ELISA Kit recognizes endogenous levels of androgen receptor protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Total Androgen Receptor Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total androgen receptor protein. An Androgen Receptor Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, androgen receptor protein is captured by the coated antibody. Following extensive washing, an Androgen Receptor Mouse Detection mAb is added to detect the captured androgen receptor protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of total androgen receptor protein. 
 Antibodies in kit are custom formulations specific to kit.

Background

Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).

1. Li, J. and Al-Azzawi, F. (2009) Maturitas 63, 142-8.
2. Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
3. Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).