DNAJC2/MPP11 (D6B1E) Rabbit mAb

• 产品编号：CST-12844S      品牌：CST       原厂货号：12844S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DNAJC2/MPP11 (D6B1E) Rabbit mAb兔单抗能够检测内源性 DNAJC2/MPP11总蛋白。该单克隆抗体经由合成的人 DNAJC2/MPP11蛋白近羧基端的氨基酸肽段免疫动物而生产的。DnaJ/Hsp40蛋白是一个含有J结构域的分子伴侣蛋白的保守家族，其可通过他们与Hsp70分子伴侣蛋白的相互作用来协助蛋白折叠和稳定（综述1）。DNAJC2,也称为MPP11 (M-phase phosphoprotein 11 protein)是核糖体相关复合物(RAC)的一个组分。RAC定位在胞质内，在这里他可以通过刺激Hsp70分子伴侣蛋白的ATPase活性来协助维持新合成多肽的正确折叠（2,3）。在核内，MPP11通过介导从polycomb抑制状态到活化的染色质的开关来参与转录激活（4）。之前的研究表明MPP11在白血病，头部及颈部癌症中过表达，致使研究者认为MPP11可能是一个潜在的治疗靶点（5-7）。

1. Qiu, X.B. et al. (2006) Cell Mol Life Sci 63, 2560-70.
2. Hundley, H.A. et al. (2005) Science 308, 1032-4.
3. Otto, H. et al. (2005) Proc Natl Acad Sci U S A 102, 10064-9.
4. Richly, H. et al. (2010) Nature 468, 1124-8.
5. Greiner, J. et al. (2003) Int J Cancer 106, 224-31.
6. Resto, V.A. et al. (2000) Cancer Res 60, 5529-35.
7. Tabarkiewicz, J. and Giannopoulos, K. (2010) Transplant Proc 42, 3293-6.

原厂资料：

Specificity / Sensitivity

DNAJC2/MPP11 (D6B1E) Rabbit mAb recognizes endogenous levels of total DNAJC2/MPP11 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DNAJC2/MPP11 protein.

Background

DnaJ/Hsp40 proteins are a conserved family of J-domain-containing chaperone proteins that assist in protein folding and stability through their interactions with Hsp70 chaperone proteins (reviewed in 1). DNAJC2, also known as MPP11 (M-phase phosphoprotein 11 protein), is a component of the ribosome-associated complex (RAC). The RAC is localized to the cytoplasm, where it assists in maintaining appropriate folding of nascent polypeptides by stimulating the ATPase activity of Hsp70 chaperone proteins (2,3). In the nucleus, MPP11 is involved in the activation of transcription through mediation of the switch from polycomb-repressed to active chromatin (4). Previous studies have shown MPP11 is overexpressed in leukemia and head and neck cancer, leading researchers to suggest MPP11 may be a potential therapeutic target (5-7).

1. Qiu, X.B. et al. (2006) Cell Mol Life Sci 63, 2560-70.
2. Hundley, H.A. et al. (2005) Science 308, 1032-4.
3. Otto, H. et al. (2005) Proc Natl Acad Sci U S A 102, 10064-9.
4. Richly, H. et al. (2010) Nature 468, 1124-8.
5. Greiner, J. et al. (2003) Int J Cancer 106, 224-31.
6. Resto, V.A. et al. (2000) Cancer Res 60, 5529-35.
7. Tabarkiewicz, J. and Giannopoulos, K. (2010) Transplant Proc 42, 3293-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.