Tunicamycin

• 产品编号：CST-12819S      品牌：CST       原厂货号：12819S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

Description

Molecular Weight:

840.9 g/mol

Molecular Characterization

Homolog A: C₃₇H₆₀N₄O₁₆

Homolog B: C₃₈H₆₂N₄O₁₆

Homolog C: C₃₉H₆₄N₄O₁₆

Homolog D: C₄₀H₆₆N₄O₁₆

Purity

>99%

原厂资料：

Description

Molecular Weight:

840.9 g/mol

Molecular Characterization

Homolog A: C₃₇H₆₀N₄O₁₆

Homolog B: C₃₈H₆₂N₄O₁₆

Homolog C: C₃₉H₆₄N₄O₁₆

Homolog D: C₄₀H₆₆N₄O₁₆

Purity

>99%

Directions for Use

Tunicamycin is supplied as a lyophilized powder. For a 5 mg/ml stock, reconstitute the 5 mg in 1 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-10 μg/ml for 0.5-20 hours.

Solubility: Soluble in DMSO, DMF or Pyridine.

Background

Isolated from Streptomyces lysosuperificus, tunicamycin is a nucleoside antibiotic that inhibits N-linked glycosylation in glycoprotein synthesis. Composed of tunicamycins A, B, C and D, the compound competitively blocks the transfer of N-acetylglucosamine-1-phosphate (GlcNAc-1-P) from UDP-GlcNAc to dolichol-P (1,2). By preventing glycoprotein synthesis, tunicamycin inhibits the formation of the “viral coat” known as the tunica or capsid in both RNA and DNA viruses, and thus exhibits antiviral properties (3). Research studies have shown that tunicamycin will arrest cells in G1 phase, preventing them from entering S-phase and increasing the expression of PERK (4,5). Following N-linked glycosylation inhibition, tunicamycin induces autophagy in response to ER stress, ultimately upregulating CHOP and BIP (6,7).

注意事项：

Storage: Store lyophilized at room temperature or in solution at
-20°C, desiccated. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles