Phospho-CaMKK2 (Ser511) Antibody

• 产品编号：CST-12818S      品牌：CST       原厂货号：12818S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-CaMKK2 (Ser511) Antibody兔多抗能够检测内源性的在Ser511位点磷酸化的CaMKK2 蛋白。该多克隆抗体是由合成的人源CaMKK2蛋白Ser511位点附近肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。>钙/钙调素依赖性蛋白激酶激酶2（CAMKK2）是钙调蛋白激酶家族的一员，包含一个中央丝氨酸/苏氨酸激酶结构域随后是一个由重叠的自抑制和CaM结合区域组成的调节结构域（1）。区别于CaMK家族其他成员，CAMKK2的ATP结合结构域中包含一个独特的脯氨酸/精氨酸/甘氨酸富集区（2）。 CAMKK2使CaMKI的177位苏氨酸和CaMK IV的200位苏氨酸磷酸化（3）。 钙离子应答时，CAMKK2也会磷酸化AMPKα（4）。 CAMKK2与长期记忆的形成（5）及脂肪细胞的发育相关（6）。 信号级联中，CAMKK2的Ser511被死亡相关蛋白激酶（DAPK）磷酸化，被认为是参与了神经细胞的死亡（7）。

1. Tokumitsu, H. et al. (1997) Biochemistry 36, 12823-7.
2. Tokumitsu, H. et al. (1995) J Biol Chem 270, 19320-4.
3. Anderson, K.A. et al. (1998) J Biol Chem 273, 31880-9.
4. Hawley, S.A. et al. (2005) Cell Metab 2, 9-19.
5. Peters, M. et al. (2003) J Neurosci 23, 9752-60.
6. Lin, F. et al. (2011) Endocrinology 152, 3668-79.
7. Schumacher, A.M. et al. (2004) Biochemistry 43, 8116-24.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Phospho-CaMKK2 (Ser511) Antibody recognizes endogenous levels of CaMKK2 protein only when phosphorylated at Ser511.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser511 of human CaMKK2 protein. Antibodies are purified by protein A and peptide affinity 

Background

Calcium/Calmodulin-dependent Protein Kinase Kinase 2 (CaMKK2) is a member of the CaMK family that contains a central Ser/Thr kinase domain followed by a regulatory domain consisting of overlapping autoinhibitory and CaM-binding regions (1). CaMKK2 can be distinguished from other CaMK family members by the presence of a unique Pro/Arg/Gly-rich insert following the ATP-binding domain (2). CaMKK2 phosphorylates CaMKI at Thr177 and CaMKIV at Thr200 (3). CaMKK2 also phosphorylates AMPKα in response to calcium (4). CaMKK2 has been implicated in long-term memory formation (5) and adipocyte development (6). CaMKK2 is phosphorylated at Ser511 by death-associated protein kinase (DAPK) in a signaling cascade thought to be involved in neuronal death (7).

1. Tokumitsu, H. et al. (1997) Biochemistry 36, 12823-7.
2. Tokumitsu, H. et al. (1995) J Biol Chem 270, 19320-4.
3. Anderson, K.A. et al. (1998) J Biol Chem 273, 31880-9.
4. Hawley, S.A. et al. (2005) Cell Metab 2, 9-19.
5. Peters, M. et al. (2003) J Neurosci 23, 9752-60.
6. Lin, F. et al. (2011) Endocrinology 152, 3668-79.
7. Schumacher, A.M. et al. (2004) Biochemistry 43, 8116-24.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody