Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb?(PE?Conjugate)

• 产品编号：CST-12812S      品牌：CST       原厂货号：12812S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

.Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb (PE Conjugate)可以检测Thr68被磷酸化的内源性Chk2蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人Chk2的 Thr68邻近残基序列一致。Cell Signaling Technology公司抗体偶联藻红蛋白（PE）并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联的Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb #2197显示同样的物种交叉反应。Chk2是出芽酵母Rad53和裂殖酵母Cds1检测点激酶的哺乳动物同源物（1-3）。Chk2的氨基端有一组七个丝氨酸或苏氨酸残基（Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, 和 Thr68）的结构，每个结构后都有一个谷氨酸（SQ或TQ基序）。这被认为是ATM/ATR激酶的最佳磷酸化位点（4,5）。在离子辐射（IR），UV辐射或羟基脲处理引起DNA损伤后，该区域的68位苏氨酸和其他位点可以被ATM/ATR磷酸化（5-7）。因此SQ/TQ结构域似乎具有调控功能。68位苏氨酸磷酸化是后续激活步骤的前提，会导致Chk2激酶结构域的383和387位苏氨酸自磷酸化（8）。

1. Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415.
2. Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.
3. Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.
4. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.
5. Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.
6. Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
7. Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
8. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb (PE Conjugate) detects endogenous levels of Chk2 only when phosphorylated at Thr68.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb #2197.

Background

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).

1. Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415.
2. Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.
3. Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.
4. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.
5. Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.
6. Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
7. Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
8. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibod-
ies. Protect from light. Do not freeze