BACH2 Antibody

• 产品编号：CST-12809S      品牌：Cell Signaling Technology       原厂货号：12809S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BACH2抗体能够检测内源性BACH2总蛋白水平。此多克隆抗体通过合成肽免疫动物制备，这种合成肽与人源BACH2蛋白Ala304周围的残基相一致。抗体通过蛋白A和多肽亲和层析纯化。转录调控因子BTB和CNC同源物2(BACH2)是一种包含bZIP域的转录抑制子，能够与MafKz形成二聚物，并结合Maf识别元素(MAREs)来调节转录(1,2)。BACH2是转录因子网络中的一部分，控制激活B细胞转化为制造抗体的浆细胞或B细胞的转型期(3-5)。浆细胞的分化需要转录因子Blimp1的参与(6)。BACH2一直活化的B细胞中Blimp1的表达，从而延迟浆细胞的分化以及为类别转换重组和高频变异提供时间(3-5)。全基因组关联研究能够将含有BACH2的基因位点与多种免疫相关异常相联系，包括I型糖尿病、克罗恩氏病和白癜风(7-10)。

1. Oyake, T. et al. (1996) Mol Cell Biol 16, 6083-95.
2. Muto, A. et al. (1998) EMBO J 17, 5734-43.
3. Muto, A. et al. (2004) Nature 429, 566-71.
4. Ochiai, K. et al. (2006) J Biol Chem 281, 38226-34.
5. Muto, A. et al. (2010) EMBO J 29, 4048-61.
6. Shaffer, A.L. et al. (2002) Immunity 17, 51-62.
7. Cooper, J.D. et al. (2008) Nat Genet 40, 1399-401.
8. Dubois, P.C. et al. (2010) Nat Genet 42, 295-302.
9. Franke, A. et al. (2010) Nat Genet 42, 1118-25.
10. Jin, Y. et al. (2012) Nat Genet 44, 676-80.

原厂资料：

Specificity / Sensitivity

BACH2 Antibody recognizes endogenous levels of total BACH2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala304 of human BACH2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The transcription regulator BTB and CNC homolog 2 (BACH2) is a bZIP domain-containing transcriptional repressor that dimerizes with MafK and binds Maf recognition elements (MAREs) to regulate transcription (1,2). BACH2 is part of a network of transcription factors that controls the transition of activated B cells into either antibody-producing plasma cells or memory B cells (3-5). Plasma cell differentiation requires the transcription factor Blimp1 (6). BACH2 suppresses expression of Blimp1 in activated B cells, which delays plasma cell differentiation and allows time for class switch recombination and somatic hypermutation (3-5). Genome-wide association studies have linked the genetic locus containing BACH2 to several immune-related disorders including type 1 diabetes, celiac disease, Crohn’s disease, and the skin condition known as vitiligo (7-10).

1. Oyake, T. et al. (1996) Mol Cell Biol 16, 6083-95.
2. Muto, A. et al. (1998) EMBO J 17, 5734-43.
3. Muto, A. et al. (2004) Nature 429, 566-71.
4. Ochiai, K. et al. (2006) J Biol Chem 281, 38226-34.
5. Muto, A. et al. (2010) EMBO J 29, 4048-61.
6. Shaffer, A.L. et al. (2002) Immunity 17, 51-62.
7. Cooper, J.D. et al. (2008) Nat Genet 40, 1399-401.
8. Dubois, P.C. et al. (2010) Nat Genet 42, 295-302.
9. Franke, A. et al. (2010) Nat Genet 42, 1118-25.
10. Jin, Y. et al. (2012) Nat Genet 44, 676-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.