K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb?(HRP Conjugate)

• 产品编号：CST-12805S      品牌：CST       原厂货号：12805S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb兔单抗 (HRP 偶联)识别在Lys48残基链接的多泛素化链。这一抗体与单泛素或其他残基链接的多泛素链无交叉反应。此单克隆抗体是通过合成与人源双泛素链Lys48残基邻近的分支序列一致的肽段，免疫动物产生的。CST公司的这个抗体通过它的胺基团与HRP的碳水化合物结合。此种HRP结合的抗体应该展现出和无偶联物的K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb #8081有相同的种属交叉反应。泛素是一种保守的多肽单元，在泛素蛋白酶体途径中起重要作用。泛素能与许多细胞蛋白共价连接，并被26S蛋白酶降解。目标蛋白泛素共价结合的进程终包含3种组分。首先泛素通过与E1泛素活化酶形成硫酯复合物而被活化。活化的泛素随后被转运到E2泛素载体蛋白，随后E3泛素连接酶把E2连接到靶蛋白的赖氨酸的epsilon位氨基上(1-3)。泛素蛋白酶体通路在正常生物过程和疾病中广泛存在。一些蛋白，诸如IκB, p53, cdc25a, 和Bcl-2等都是泛素-蛋白酶体途径的靶蛋白，并可以调节细胞周期进展、分化、细胞应激反应以及凋亡(4-7)。

1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
3. Hochstrasser, M. (2000) Science 289, 563-4.
4. Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
5. Aberle, H. et al. (1997) EMBO J 16, 3797-804.
6. Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
7. Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.

原厂资料：

Specificity / Sensitivity

K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb (HRP Conjugate) detects polyubiquitin chains formed by Lys48 residue linkage. This antibody does not react with monoubiquitin or polyubiquitin chains formed by specific linkage to a different lysine residue.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the Lys48 branch of the human diubiquitin chain.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb #8081.

Background

Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH₂ of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).

1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
3. Hochstrasser, M. (2000) Science 289, 563-4.
4. Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
5. Aberle, H. et al. (1997) EMBO J 16, 3797-804.
6. Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
7. Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibodies.