GLDC Antibody

• 产品编号：CST-12794S      品牌：CST       原厂货号：12794S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GLDC抗体可以识别内源性总的GLDC蛋白。多抗由合成肽段免疫动物产生，该肽段与人GLDC蛋白Val668附近氨基酸一致。抗体由蛋白A和肽段亲和层析技术纯化得到。甘氨酸脱羧酶（GLDC）是线粒体蛋白复合物的一个成分，其催化甘氨酸的降解(1)。甘氨酸小肠系统由三个不同的酶 (P-, T- 和 L-蛋白)和另一个组分(H蛋白)组成，能够把一个甘氨酸甲胺组从一个酶转移到另一个。GLDC蛋白(P-蛋白)是一个脱羧酶能够为甲胺组转移T-蛋白提供帮助(2)。非小细胞肺癌(NSCLC)中的肿瘤起始细胞表达高水平的GLDC和LIN28B，这两者都对肿瘤起始细胞增殖过程非常重要(3)。GLDC是一个致癌基因们通过其代谢能力促进肿瘤发生(3)。GLDC基因突变是多数甘氨酸脑病的病因，这是一种代谢疾病其特点是甘氨酸的积累，嗜睡，肌张力低下，顽固性癫痫发作和死亡(4).

1. Kume, A. et al. (1991) J Biol Chem 266, 3323-9.
2. Kanno, J. et al. (2007) J Med Genet 44, e69.
3. Zhang, W.C. et al. (2012) Cell 148, 259-72.

原厂资料：

Specificity / Sensitivity

GLDC Antibody recognizes endogenous levels of total GLDC protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val668 of human GLDC protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Glycine decarboxylase (GLDC) is a component of a mitochondrial protein complex that catalyzes the degradation of glycine (1). The glycine cleavage system is composed of three distinct enzymes (P-, T- and L-proteins) and an additional component (H-protein) that transfers a glycine methylamine group from one enzyme to another. The GLDC protein (P-protein) is the decarboxylase that binds the methylamine group for transfer to the T-protein (2). Tumor-initiating cells in the primary non-small cell lung cancer (NSCLC) express high levels of GLDC and LIN28B, both of which are essential for the proliferation of tumor-initiating cells (3). GLDC is an oncogene that promotes tumorigenesis through its metabolic activity (3). Mutations in the corresponding GLDC gene account for the majority of reported cases of glycine encephalopathy, which is a metabolic disorder characterized by the accumulation of glycine, lethargy, hypotonia, intractable seizures, and death (4).

1. Kume, A. et al. (1991) J Biol Chem 266, 3323-9.
2. Kanno, J. et al. (2007) J Med Genet 44, e69.
3. Zhang, W.C. et al. (2012) Cell 148, 259-72.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.