Human Interleukin-6 Receptor α (hIL-6Rα)

• 产品编号：CST-12786SC      品牌：CST       原厂货号：12786SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 核受体蛋白
• 应用分类：

描述：

Source / Purification

Recombinant Human Interleukin-6 Receptor α (hIL-6Rα) Leu20 - Asp358 (Accession #NP_000556) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Molecular Characterization: Recombinant hIL-6Rα has a calculated MW of 37,871. DTT-reduced and non-reduced protein migrate as 66 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal LAPRR of recombinant hIL-6Rα was verified by amino acid sequencing.

Purity

>90% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hIL-6Rα. All lots are greater than 90% pure.

Bioactivity

The activity of hIL-6Rα was determined by assessing its ability to enhance IL-6 mediated inhibition of M1 cells proliferation. The ED₅₀ of each lot is between 2 and 15 ng/ml.

原厂资料：

Source / Purification

Recombinant Human Interleukin-6 Receptor α (hIL-6Rα) Leu20 - Asp358 (Accession #NP_000556) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Molecular Characterization: Recombinant hIL-6Rα has a calculated MW of 37,871. DTT-reduced and non-reduced protein migrate as 66 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal LAPRR of recombinant hIL-6Rα was verified by amino acid sequencing.

Purity

>90% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hIL-6Rα. All lots are greater than 90% pure.

Bioactivity

The activity of hIL-6Rα was determined by assessing its ability to enhance IL-6 mediated inhibition of M1 cells proliferation. The ED₅₀ of each lot is between 2 and 15 ng/ml.

Applications

Optimal concentration for the desired application should be determined by the user.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-6Rα.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of hIL-6Rα in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg

hIL-6Ra. Carrier free: Lyophilized from a 0.22 μm filtered solution of hIL-6Rα in 20 mM Tris, pH 7.2.

Background

The IL-6 receptor is a heterodimeric complex that consists of a ligand-binding IL-6 receptor α (IL-6Rα) subunit and a signaling component, gp130 (1). Binding of IL-6 to IL-6Rα results in dimerization of receptor with gp130 and subsequent STAT3 activation (1). IL-6Rα is cleaved from the cell surface by ADAM17 (1,2). In humans, soluble IL-6Rα is also generated via alternatively spliced mRNA (1,3). Soluble IL-6Rα binds to IL-6 and can stimulate signaling via membrane bound gp130 in a process known as “trans-signaling” (1). It is through trans-signaling that IL-6 stimulates cells that do not express membrane bound IL-6Rα (1).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20ºC should be in a manual defrost freezer