NCK1 (5B7) Mouse mAb

• 产品编号：CST-12778S      品牌：CST       原厂货号：12778S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

</p> NCK1 (5B7) Mouse mAb可以识别内源性总的NCK1蛋白。</p>单克隆抗体由合成重组人NCK1蛋白片段免疫动物产生。</p>NCK1（也被称为NCK或NCKα）是一个广泛表达的肿瘤接头蛋白包括了三个SH3结构域和一个SH2结构域（1-3）。NCK1会在多种细胞表面受体作用下被磷酸化，涉及多种刺激引起的肌动蛋白丝细胞骨架构建（4-6）。NCK2（也被称为NCKβ），是NCK1的同源蛋白，与NCK1有重合的表达谱和功能（7）。

1. Lehmann, J.M. et al. (1990) Nucleic Acids Res. 18, 1048.
2. Chou, M.M. et al. (1992) Mol. Cell Biol. 12, 5834-5842.
3. Li, W. et al. (1992) Mol. Cell Biol. 12, 5824-5833.
4. Verma, R. et al. (2006) J. Clin. Invest. 116, 1346-1359.
5. Rivera, G.M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9536-9541.
6. Li, H. et al. (2006) Biochem. Biophys. Res. Commun. 349, 310-316.
7. Bladt, F. et al. (2003) Mol. Cell Biol. 23, 4586-4597.

原厂资料：

Specificity / Sensitivity

<p>NCK1 (5B7) Mouse mAb recognizes endogenous levels of total NCK1 protein.

Source / Purification

<p>Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to a fragment of human NCK1 protein.

Background

<p>NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).

1. Lehmann, J.M. et al. (1990) Nucleic Acids Res. 18, 1048.
2. Chou, M.M. et al. (1992) Mol. Cell Biol. 12, 5834-5842.
3. Li, W. et al. (1992) Mol. Cell Biol. 12, 5824-5833.
4. Verma, R. et al. (2006) J. Clin. Invest. 116, 1346-1359.
5. Rivera, G.M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9536-9541.
6. Li, H. et al. (2006) Biochem. Biophys. Res. Commun. 349, 310-316.
7. Bladt, F. et al. (2003) Mol. Cell Biol. 23, 4586-4597.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.