Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit

• 产品编号：CST-12775S      品牌：Cell Signaling Technology       原厂货号：12775S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit中的每种抗体都能够检测内源性特异目的蛋白的总水平。CHD8 (D3C1) Rabbit mAb 兔单抗还能与一个140 kDa的未知来源蛋白发生交叉反应。单克隆抗体是采用合成的多肽免疫动物而生产的，对应序列信息如下：人CHD1蛋白近羧基末端，人CHD7蛋白的氨基末端或人CHD8蛋白的羧基端，。多克隆抗体是经由合成的人CHD3蛋白、CHD4蛋白或围绕人CHD2的 Phe1579残基的多肽免疫动物而生产的。多克隆抗体由蛋白质A和多肽亲和层析纯化而成。使用Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit可以一种快速而经济的方法来检测与组蛋白去乙酰化酶NuRD复合体相互租用的CHD家族成员。试剂盒提供的每种一抗都足够进行4次western blot实验。Chromodomain-helicase-DNA-binding domain (CHD)蛋白已经在多种生物中被鉴定(1,2)。由九个成员组成的蛋白家族已经被分为三个亚家族：subfamily I (CHD1和CHD2)、subfamily II (CHD3和CHD4)和subfamily III (CHD5、CHD6、CHD7、CHD8、CHD9)。所有CHD蛋白包含两个串联的N端chromodomains、一个SWI/SNF相关的ATPase结构域和一个C端DNA结合区域 (1,2)。chromodomains有助于结合到组蛋白的已甲基化赖氨酸残基，并且可与染色质的特异区域相互作用。SWI/SNF相关的ATPase区域可利用来自ATP水解的能量去修饰染色质结构。CHD蛋白经常发现在大的多蛋白复合物中，伴随着转录激活或抑制活性，这有该复合物中其它蛋白质监管。CHD3 (也称为Mi2-α)和CHD4 (也称为Mi2-β)是核小体重塑和组蛋白去乙酰化酶(NuRD)转录抑制复合物的中心成员，其包含HDAC1、HDAC2、RbAp48、RbAp46、MTA1、MTA2、MTA3和MBD3 (3-8)。CHD3和CHD4包含两个PHD (plant homeodomain)锌指结构，该结构直接结合到HDAC1和HDAC2。

1. Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
3. Tong, J.K. et al. (1998) Nature 395, 917-21.
4. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
5. Zhang, Y. et al. (1998) Cell 95, 279-89.
6. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
7. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
8. Fujita, N. et al. (2003) Cell 113, 207-19.

原厂资料：

Specificity / Sensitivity

Each antibody in the Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit recognizes total endogenous levels of the specific target protein. CHD8 (D3C1) Rabbit mAb also cross-reacts with a protein of unknown origin at 140 kDa.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human CHD1 protein, the amino terminus of human CHD7 protein or the carboxy terminus of human CHD8 protein. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to human CHD3 protein, human CHD4 protein or residues surrounding Phe1579 of human CHD2 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit provides an economical means to investigate the CHD family members associated with the histone deacetylase NuRD complex. This kit contains enough primary antibody to perform four western blots per primary antibody.

Background

Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of nine proteins is divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, and CHD9). All CHD proteins contain two tandem amino-terminal chromodomains, a SWI/SNF-related ATPase domain, and a carboxy-terminal DNA-binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes energy from ATP hydrolysis to modify chromatin structure. CHD proteins are often found in large, multiprotein complexes with their transcriptional activation or repression activity governed by other proteins within the complex. CHD3 (also known as Mi2-α) and CHD4 (also known as Mi2-β) are central components of the nucleosome remodeling and histone deacetylase (NuRD) transcriptional repressor complex, which also contains HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (3-8). Both CHD3 and CHD4 contain two plant homeodomain (PHD) zinc finger domains that bind directly to HDAC1 and HDAC2.

1. Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
3. Tong, J.K. et al. (1998) Nature 395, 917-21.
4. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
5. Zhang, Y. et al. (1998) Cell 95, 279-89.
6. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
7. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
8. Fujita, N. et al. (2003) Cell 113, 207-19.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.
Recommended Antibody Dilutions:
Western blotting  1:1000