RBBP5 (D4Y7R) Rabbit mAb

• 产品编号：CST-12766S      品牌：CST       原厂货号：12766S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RBBP5 (D4Y7R) recombinant antibody能够检测内源性RBBP5总蛋白水平。该单克隆抗体是采用合成的与人源RBBP5蛋白Gly61残基周围序列相对应的肽段免疫动物而生产的。Set1组蛋白甲基转移酶最初是在酵母中被首次鉴定出来的，并且是Set1/COMPASS组蛋白甲基转移酶复合物中的一部分，该复合物能偶使histone H3蛋白Lys4位点甲基化并作为转录辅助激活因子起作用(1)。酵母仅含有一个已知的Set1蛋白，而哺乳动物则含有六个Set1相关蛋白：SET1A、SET1B、MLL1、MLL2、MLL3和MLL4，所有这些蛋白都能组装到COMPASS样复合物中，并使组蛋白H3 的Lys4位点甲基化(2,3)。这些Set1相关蛋白中的每一个都是在不同的蛋白质复合物被发现的，他们都含有共同亚基WDR5、RBBP5、ASH2L、CXXC1和DPY30，而这些亚基是复合物的正确组装和组蛋白甲基转移酶活性调节所需要的(2-6)。MLL1和MLL2复合物含有另外一个蛋白亚基menin (6)。像酵母Set1一样，所有六种Set1相关哺乳动物蛋白可以使组蛋白 H3 Lys4位点甲基化(2-6)。许多血液恶性肿瘤种都发现有MLL易位，这暗示着Set1/COMPASS组蛋白甲基转移酶复合物在白血病生成中有至关重要的作用 (6)。

1. Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
2. Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
3. Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
4. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
5. Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
6. Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Zebrafish, Horse

Specificity / Sensitivity

RBBP5 (D4Y7R) recombinant antibody recognizes endogenous levels of total RBBP5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly261 of human RBBP5 protein.

Background

The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30, which are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6).

1. Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
2. Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
3. Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
4. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
5. Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
6. Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody