PiT1/SLC20A1 (D1Z4X) Rabbit mAb

• 产品编号：CST-12765S      品牌：CST       原厂货号：12765S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PiT1/SLC20A1(D1Z4X) Rabbit mAb兔单抗可以识别内源性PiT1总蛋白水平。单克隆抗体由合成肽段免疫动物产生，该肽段与人PiT1蛋白Gly290附近氨基酸序列一致。磷酸盐转运蛋白1(PiT1/SLC20A1)是一个钠依赖性磷酸(Pi)转运子能够将Pi转入细胞。PiT1最初被认为是逆转录病毒(1,2)。它在多种组织中广泛表达，在维持细胞Pi稳态过程中发挥了关键作用(3,4)。不依赖于PiT1磷酸盐转运功能，磷酸盐转运蛋白1对细胞增殖和肿瘤细胞生长非常重要(5)。研究人员发现PiT1参与了TNF-α诱导的细胞凋亡(6)。此外，通过PiT1摄入磷酸盐对于血管钙化至关重要(7)，过表达PiT1会导致Werner 综合征病人软组织钙化(8)。更多的研究指出 钙化性主动脉瓣疾病人(CAVD) 组织中PiT1表达量上调，PiT1通过修饰Akt1水平增强细胞凋亡和钙化(9)。

1. O'Hara, B. et al. (1990) Cell Growth Differ 1, 119-27.
2. Miller, D.G. et al. (1994) Proc Natl Acad Sci U S A 91, 78-82.
3. Kavanaugh, M.P. et al. (1994) Proc Natl Acad Sci U S A 91, 7071-5.
4. Uckert, W. et al. (1998) Hum Gene Ther 9, 2619-27.
5. Beck, L. et al. (2009) J Biol Chem 284, 31363-74.
6. Salaün, C. et al. (2010) J Biol Chem 285, 34408-18.
7. Li, X. et al. (2006) Circ Res 98, 905-12.
8. Honjo, S. et al. (2008) Rejuvenation Res 11, 809-19.
9. El Husseini, D. et al. (2013) PLoS One 8, e53393.

原厂资料：

Specificity / Sensitivity

PiT1/SLC20A1(D1Z4X) Rabbit mAb recognizes endogenous levels of total PiT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly290 of human PiT1 protein.

Background

Phosphate transporter 1 (PiT1/SLC20A1) is a sodium dependent phosphate (Pi) transporter that imports Pi into cells. PiT1 was initially identified as a receptor for retroviruses (1,2). It is widely expressed in various tissues where it plays a critical role in maintaining cellular Pi homeostasis (3,4). Phosphate transporter 1 is important in cell proliferation and tumor cell growth independent of PiT1 phosphate transport function (5). Researchers have found that PiT1 is involved in TNF-α induced apoptosis (6). Moreover, phosphate uptake via PiT1 is crucial for vascular calcification (7) and overexpression of PiT1 leads to soft tissue calcification in Werner syndrome patients (8). Additional research indicates that increased PiT1 expression is seen in calcific aortic valve disease (CAVD) tissues, and that PiT1 enhances apoptosis and mineralization by modifying Akt1 levels (9).

1. O'Hara, B. et al. (1990) Cell Growth Differ 1, 119-27.
2. Miller, D.G. et al. (1994) Proc Natl Acad Sci U S A 91, 78-82.
3. Kavanaugh, M.P. et al. (1994) Proc Natl Acad Sci U S A 91, 7071-5.
4. Uckert, W. et al. (1998) Hum Gene Ther 9, 2619-27.
5. Beck, L. et al. (2009) J Biol Chem 284, 31363-74.
6. Salaün, C. et al. (2010) J Biol Chem 285, 34408-18.
7. Li, X. et al. (2006) Circ Res 98, 905-12.
8. Honjo, S. et al. (2008) Rejuvenation Res 11, 809-19.
9. El Husseini, D. et al. (2013) PLoS One 8, e53393.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.